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題 名 | 利用cDNA微陣列來建構一個分子生物層級的中草藥分析平臺=Constructing a Chinese Medicine Molecular Analysis Plateform by Utilizing cDNA Microarray |
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編 次 | 3 |
作 者 | 許志楧; | 書刊名 | 中醫藥年報 |
卷 期 | 25:2 2007.10[民96.10] |
頁 次 | 頁339-371 |
專 輯 | 中醫藥基因體及免疫學研究 |
分類號 | 414.33 |
關鍵詞 | 冬蟲夏草; cDNA微陣列; 分子基因圖譜; 化學指紋圖譜; 標記基因群; Cordyceps sinensis; cDNA microarray; Molecular genetic fingerprinting; Chemical fingerprinting; Marker genes; Standardization; |
語 文 | 中文(Chinese) |
中文摘要 | 中醫藥已經有數千年的使用經驗,其療效也記載於各種中藥的古籍之中。中藥的精華之一是使用複方來治療疾病,然而即使是單方也含有數以百計的有機與無機化合物。如果缺乏標準化的鑑定,吾人將無法更有效、安全的使用中草藥。因此,我們需建立一個標準化的程序,來鑑定中草藥的品種以及批次間的化學與生物活性的一致性。 本研究第三年,依據先前的經驗進一步更仔細的對冬蟲夏草萃取液的有機化學圖譜(Organic chemical fingerprint)進行分析,得到更完整的有機化學指紋圖譜。並以統計分析來區分各種樣本間的相似程度。對於微陣列指紋圖譜(Microarray fingerprint)也以統計的方法確認資料的信度以及進一步篩選出冬蟲夏草標記基因群(Marker genes)。為了以實驗驗證微陣列結果的可信度,我們進行同步定量聚合反應(Real-time quantitative PCR),而且以統計的分析來比較此兩種結果之間的一致性。 |
英文摘要 | The traditional Chinese medical formulas have been in clinical use for thousands of years and their therapeutic effects were documented in ancient Chinese pharmacopoeias. One of the salient features of the traditional Chinese medicine is the use of combination herbal formula. However, even a single medicinal herb contains hundreds of organic and inorganic compounds. Lack of standardization poses a major challenge for the safe and effective use of traditional Chinese herbal mdeicine. Therefore, it is essential to establish a standardization protocol to ensure the authenticity as well as assess the chemical and biological consistency of these multi-component herbal formulas. In the third year, we have performed a detailed investigation on the chemical profiles of Cordyceps extracts. Final chemical fingerprints of various Cordyceps samples are constructed. In addition, microarray results are further confirmed by real-time PCR and the consistency between these two sets of data are evaluated by statistical methods. As the end of the third year, we have demonstrated that an intergrated fingerprinting platform is established. This platform holds potential for future application on the study of traditional Chinese herbal formulas. |
本系統中英文摘要資訊取自各篇刊載內容。