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題 名 | 半夏炮製技術之研究(1)=The Study of Process Technology of Pinellia Ternate (Thunb.) Breit. (1) |
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作 者 | 溫武哲; | 書刊名 | 中醫藥年報 |
卷 期 | 24:3 民95.10 |
頁 次 | 頁1-39 |
專 輯 | 中醫藥科技化相關研究 |
分類號 | 414.4 |
關鍵詞 | 藥材炮製; 清半夏; 薑半夏; 急性毒性; 細胞毒性; 品質管制; Herb processing; Water processed pinellia ternate; Ginger processed pinellia ternate; Acute toxicity; LD50; Cytotoxicity; Quality-control; |
語 文 | 中文(Chinese) |
中文摘要 | 本計畫進行半夏藥材炮製方法、炮製前後化學成分變化化學指紋圖譜、大鼠急性毒性(LD50)及細胞毒性試驗研究分析。 在炮製方法研究方面進行了:1. 半夏藥材基原鑑定研究,以組織刀片及顯微鏡檢確定半夏藥材正確基原,再進行藥材炮製;2. 炮製方法篩選,依據古籍記載炮製方法進行炮製,最後篩選出最適炮製方法--清半夏:篩選1~1.5cm經基原確立之生半夏藥材,以藥材5倍體積量之1%礬水浸泡,每天更換1%礬水,共七天,以50℃烘乾48小時得清半夏;薑半夏--取經前述方法炮製完成之清半夏,以2.5倍藥材水量煮5小時,切成1-1.5mm薄片,再以30℃冷風烘乾或風乾48小時,其LOD值為8~12%,得薑半夏成品。在半夏炮製前後化學變化研究,開發HPLC分析方法及分析半夏藥材炮製過程中成份變化指紋圖譜,研究結果顯示,炮製7天後之半夏藥材,其HPLC圖譜中,40分鐘前中高級性化學成分減少消失,顯示炮製過程中高級化學成分會被去除。在毒性部分研究,分成兩部份,大鼠急性毒性(LD50)試驗與細胞毒性試驗。於大鼠LD50試驗中結果數據顯示,不論生半夏或炮製7天後清半夏,其對大鼠動物均無急性致死劑量(10g/kg),而細胞試驗(BHK-21)結果亦顯示對此細胞並無毒性,有待進一步評估,以確定其毒性。 本研究成果可提供生產品質穩定之清半夏和薑半夏,並可提供半夏藥材炮製流程方法規格化及科學化之參考依據。所建立之品質管控方法,可提供制定炮製方法規範之參考,對於促進此藥材之現代化與國際化有相當之助益。 |
英文摘要 | The objective of research is to study the process technology of Pinellia ternate (Thunb.) Breit., the fingerprint of Pinellia ternate before and after processing, the acute toxicity of Pinellia ternate to the rats (LD50), and the cytotoxic effects of Pinellia ternate to BHK-21 cells. Before the study, we had identified the orginal of Pinellia ternate, via the methods of histology and microscopy analysis. At the process technology study, process Pinellia ternate, then to screen the best methods of producing “Water processed Pinellia ternate” were prepared by using 1~1.5cm of Pinellia ternate which had already been original identification, bathed with 5 times volume of 1% alum water solution, replaced with 1% alum water solution every day for constinuously seven days, and dried at 50℃ for 48 hours. “Ginger processed Pinellia ternate” were prepared by using “water processed Pinellia ternate”, boiled with 2.5 times volume of water solution which contained ginger and alum, then were cut as 1-1.5mm slice, and dried at 30℃ for 48 hours (LOD 8~12%). At the chemistry study of processed Pinellia ternate, we had developed the HPLC analysis methods and analyzed the fnigerprint of Pinellia ternate before and after processing. Our results showed that, after seven days of Pinellia ternate processing, at HPLC fingerprint profile, there are some kinds of chemical ingredients disappeared. The toxicity study of processed Pinellia ternate included the acute toxicity of Pinellia ternate to the rats (LD50) and the cytotoxic effects of Pinellia ternate to BHK-21 cells. The data showed that there are no significant difference of the rat's acute toxicity (10g/kg) between “Pinellia ternate” and “water processed Pinellia ternate”. To evaluate the cytotoxic effects of Pinellia ternate, we still need more dat to confirm our results. Our study will provide the methods for processing stable quality of “water processed Pinellia ternate” and “ginger processed Pinellia ternate”. It will also contribute as the standard and scientific reference for the methods of processing of Pinellia ternate. These quality-controlled methods wil not only give us an example of how to set up the processing model of Pinellia ternate, will be also beneficial to the modernization and internalization of Pinellia ternate. |
本系統中英文摘要資訊取自各篇刊載內容。