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題名 | 防風指標成分之製備與品質管制=The Marker Standard Preparation of Saposhnikoviae Radix and Quality Control |
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作 者 | 楊玲玲; | 書刊名 | 中醫藥年報 |
卷期 | 17:2 1999.05[民88.05] |
頁次 | 頁499-512 |
分類號 | 414.3 |
關鍵詞 | 防風; 指標成分; 品質管制; 市售品; Saposhnikovia divaricata; Cimifugin; Marker substance; Quality control; Commercial crude drug; |
語文 | 中文(Chinese) |
英文摘要 | Cimifugin, one of the analgesic and anti-hypertension principle component of Fang-Feng, was isolated from the root of Saposhnikovia divaricata. Reversed-phase high-performance liquid chromatography was employed to determine the contents of cimifugin in Fang-Feng. The separation was performed on a LiChrospher 100 RP-18e column (4 mm I.D.X 250 mm) by isocretic elution with acetonitrile-water (v/v) (18:82) as the mobile phase at a flow-rate of 1.0 ml/min, the temperature keep at 40 ℃ and with detection at 220 nm. Methylparaben was used as the internal standard and a regression equation revealed linear relationship between the peak-area ratio (marker substance/internal standard) and concentration. We found that the decoction from Fang-Feng with 20 times of 70% aqueous methanoi in 40 minutes of sonication given the best tield of cimifugin. This HPLC method can rapidly and simply quantify the cimifugin present in Fang-Feng and can be used for quality control of commercial crude drug of Fang-Feng. |
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