查詢結果分析
來源資料
相關文獻
- 臺灣土雞生殖細胞中Vasa基因的表現分析
- Malignant Primary Extragonadal Germ Cell Tumors of Mediastinum--An Analysis of Clinical and Radiological Features in 15 Cases
- Primary Nonseminomatous Germ Cell Tumors of the Mediastinum
- Mixed Germ Cell Tumor Presenting as Intratumoral Hemorrhage: Report of a Case Originated from the Pineal Region
- Clinical Experiences of Germ Cell Tumor in Cryptorchid Testis
- 人類幹細胞研究的法議題
- ES細胞.EG細胞vs.法律
- Mixed Germ Cell Tumor Arising in Complete Androgen Insensitivity Syndrome, Associated with Mullerian Structures
- 一個跨代的生命倫理思考:生殖細胞基因治療的規範形成
- Growing Teratoma Syndrome: A Case Report
頁籤選單縮合
題 名 | 臺灣土雞生殖細胞中Vasa基因的表現分析=Expression Analysis of Vasa Gene in Germ Cells of Native Taiwan Chickens |
---|---|
作 者 | 蕭振文; 蔡麗卿; 劉瑞珍; 戴謙; 劉振發; 陳立人; | 書刊名 | 畜產研究 |
卷 期 | 39:2 民95.06 |
頁 次 | 頁121-129 |
分類號 | 437.713 |
關鍵詞 | 生殖細胞; 即時聚合酶連鎖反應; Vasa基因; Germ-cells; Gene analysis; Vasa gene; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究目的在應用反轉錄-聚合酶連鎖反應(reverse transcription-polymerase chain reaction, RT-PCR)分析臺灣土雞組織vasa基因的mRNA表現。同時將vasa基因的mRNA表現。同時將vasa基因之PCR產物進行DNA序列分析,以確認vasa基因表現之正確性。將臺灣土雞之睪丸、卵巢、始基生殖細胞(primordial germ cells, PGCs)、胸肌、心臟與肝臟等組織,應用套組萃取樣品的mRNA後,進行反轉錄以合成互補DNA,再設計引子分析樣品中vasa基因的表現,同時以Gapdh基因做為分析之陽性對照。不同樣品之cDNA利用引子進行預先擴增後,再以nested引子進行第二次即時PCR(real-time PCR)偵測。試驗結果顯示,vasa僅表現在生殖細胞譜系的睪丸,卵巢與PGCs,而不表現在其他不同臟器組織。本研究結果顯示,vasa基因的表現具有組織專一性,可作為鑑定臺灣土雞生殖細胞譜系的特異分子標記。 |
英文摘要 | The purpose of this study was to investigate the transcription of vasa gene in gonads, PGCs, and various organs collected from Taiwan native chickens. The mRNAs from samples were purified and the cDNA was obtained by the reverse transcription-polymerase chain reaction (RT-PCR) to measure the expression of vasa gene. In order to further verify these PCR products, the real-time PCR products specific to vasa was subjected to DNA sequencing. The expression of Gapdh housekeeping gene was detected fro positive control. The results showed that the expression of vasa gene could only be detected in testes, ovaries and PGCs, but not in muscle, heart of liver of Taiwan Native Chickens. The results demonstrated that RT-PCR technique was a valuable tool for the analysis of important genes in germline lineages. This system could be used in the development of reliable molecular markers for screening avian germ-cell lineage. |
本系統中英文摘要資訊取自各篇刊載內容。