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頁籤選單縮合
題 名 | An Improved Procedure for the Pulsed-field Gel Electrophoresis Analysis of Vibrio Vulnificus=改進創傷弧菌脈衝式電泳分型方法 |
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作 者 | 黃顯宗; 郭政儒; | 書刊名 | 藥物食品分析 |
卷 期 | 14:2 2006.06[民95.06] |
頁 次 | 頁215-217+222 |
分類號 | 414.83 |
關鍵詞 | 創傷弧菌; 脈衝式電泳; 核酸分解; Vibrio vulnificus; Pulsed-field gel electrophoresis; DNase; |
語 文 | 英文(English) |
中文摘要 | 創傷弧菌是海洋中的致病性弧菌,經由傷口感染成遭遇水產食物傳染,所造成的敗血症死亡率高。脈衝式電泳分型是弧菌良好的分子生物分裂方法,然而在創傷弧菌中高速12%菌株的分析受阻於染色體核酸的分解。本研究提供了兩項改良,增進分析成效。其一是先破壞外膜,清除週質的成分。其二是延長蛋白質酵素作用,清除分解性的蛋白質。實驗結果顯示八株中的五株可以得到清晰的分型圖譜,顯示本改良方法得到顯著的改善成果,益可作為創傷弧菌或其他弧菌分型分析之參考。 |
英文摘要 | Vibrio vulnificus is a marine bacterium that causes septicemia with high mortality via wound infection or seafood ingestion. Subspecies typing of V. vulnificus can be performed by pulsed-field gel electrophoresis (PFGE). However, this method is hampered by the degradation of chromosomal DNA in about 12% of strains. This study presents a modified PFGE procedure. The outer membrane of V. vulnificus cells was lysed utilizing a sucrose-EDTA method and components in the periplasmic space were removed prior to making plugs. Cytoplasmic digestive enzymes were inactivated by an extended proteinase K reaction. Experimental results indicated that five out of eight strains exhibiting smeared DNA in a previous study (Appl. Environ. Microbiol. 70: 5153-5158. 2004) achieved clear banding patterns. This modified PFGE procedure can be applied to improve PFGE typing of untypeable strains of V. vulnificus. |
本系統中英文摘要資訊取自各篇刊載內容。