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題名 | 馴化光度對白馬粗肋草組織培養苗出瓶後葉片光合作用與植株生長之影響=Effects of Photosynthetic Photon Flux during ex vitro Acclimatization on Photosynthetic Behaviors and Growth of Micropropagated Aglaonema ‘White Tip’ Plantlets |
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作者 | 褚昱均; 葉德銘; | 書刊名 | 臺灣園藝 |
卷期 | 52:2 民95.06 |
頁次 | 頁199-208 |
分類號 | 435.13 |
關鍵詞 | 粗肋草; 光度; 馴化; 蒸散作用; 氣孔導度; Aglaonema; Photosynthetic photon flux density; Acclimatization; Transpiration stomatal conductance; |
語文 | 中文(Chinese) |
中文摘要 | 白馬粗肋草組織培養苗出瓶置於 25±1℃ 之育苗室,每日分別處理16小時之 80、130 或 200~μmol m¯²s¯¹ 光度馴化 70 天,再經溫室栽培 90 天調查後期生長狀況,結果顯示出瓶後以130 μmol m¯²s¯¹ 處理者生長表現最佳。瓶內生長葉片之 Fv/Fm 值,在剛出瓶時為0.79,出瓶 1 天時下降至 0.64,以 80 或 130 μmol m¯²s¯¹ 處理者之測值皆在第 2 天時即回升,之後回復至正常範圍;但以 200 μmol m¯²s¯¹ 處理者,瓶內形成之葉片在處理約第 3 週時與瓶外形成之葉片 Fv/Fm 值皆降至正常範闇值以下。以 80 μmol m¯²s¯¹ 處理者,其瓶外形成葉片之光量子產量與瓶內外葉片之淨光合作用速率皆低於以 130 μmol m¯²s¯¹ 處理者。以 200 μmol m¯²s¯¹ 處理之瓶外新生葉片,其光量子產量與光化學淬熄係數皆較低,而最低螢光量較高。組培苗之蒸散速率和氣孔導度測值,在剛出瓶時皆很高,出瓶 1 天之測值即急速下降。在瓶內形成葉片的二氧化碳交換速率在剛出瓶時約為 1.6 μmol CO2 m¯²s¯¹ ,於出瓶 3 天時較低,之後以 80 和 130 μmol m¯²s¯¹ 處理者即呈現上升趨勢;但以200 μmol m¯²s¯¹ 處理者,在出瓶 35 天內 CO2 交換速率持續下降。 |
英文摘要 | Micropropagated Aglaonema 'White Tip' plantlets were transplanted to a soilless mix and placed in a growth room at 25±1℃ under cool-white fluorescent light providing 16 h daily photosynthetic photon flux of 80, 130 or 200 μmol m¯²s¯¹ . After having been acclimatized for 70 days in the growth room, the plantlets were then transferred to a shaded greenhouse for 90 days and their growth was measured. Plantlets under 130 μmol m¯²s¯¹ had subsequent greater growth. In all plantlets tested, the Fv/Fm value of in vitro formed leaves was measured as 0.79 at time of transferring out of flasks and then decreased to 0.64 on day 1. This Fv/Fm value increased to 0.75 - 0.85 in plantlets under 80 or 130 μmol m¯²s¯¹ from day 2. In contrast, the Fv/Fm values were below 0.7 in both in vitro leaves and ex vitro leaves on day 21 in plantlets under 200 μmol m¯²s¯¹. Plantlets under 80 μmol m¯²s¯¹ had lower quantum yield of in vitro leaves and net photosynthesis of ex vitro leaves than plantlets under 130 μmol m¯²s¯¹. Ex vitro formed leaves in plantlets under 200 μmol m¯²s¯¹ had lowest quantum yield and qP, and highest F0 value. For all the plantlets tested, the stomatal conductance and transpiration rate values in the in vitro formed leaf were high at transfer and decreased dramatically on day 1 after transfer. Photosynthetic rate of the in vitro formed leaf was measured as 1.6 μmol m¯²s¯¹ C02 at transfer, decreased on day 3 and increased in plantlets under 80 or 130 μmol m¯²s¯¹ within 40 days after transfer, but decreased gradually in those under 200 μmol m¯²s¯¹ during the 35 days after transfer. |
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