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題名 | 藉由分析核糖體核酸內轉錄間隔區探討臺灣原生石斛蘭之親緣關係=Determination of the Genetic Relationship of Dendrobium spp. (Orchidaceae) in Taiwan Based on the Sequence of the Internal Transcribed Spacer of Ribosomal DNA |
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作者 | 蔡奇助; 黃柄龍; 蘇育彥; Tsai, Chi-chu; Huang, Ping-long; Su, Yu-yen; |
期刊 | 高雄區農業改良場研究彙報 |
出版日期 | 20050300 |
卷期 | 16:1 民94.03 |
頁次 | 頁13-27 |
分類號 | 377.2102 |
語文 | chi |
關鍵詞 | 石斛蘭; 親緣關係; 核糖體核酸; 內轉錄間隔區; Dendrobium; Phylogeny; rDNA; ITS; |
中文摘要 | 本研究藉由分析核糖體核酸(ribosomal DNA, rDNA)內轉錄間隔區(internal transcribed spacer, ITS)之序列,以探討台灣12 種原生石斛蘭的親 緣關係。將所分析之樣本的ITS 序列進行比較排列後, 以Kimura 2-parameters 換算物種兩兩間的遺傳距離(genetic distance),經不加權平均 重法(unweighted pair-group method analysis, UPGMA)進行群叢分析並完成親緣關係樹狀圖(phylogenetic tree)。根據樹狀圖所呈現之物種間的親緣關係, 台灣的原生石斛蘭主要可以分成四群, 其中櫻石斛(Dendrobium linawianum)、白花石斛(D. moniliforme)、黃花石斛(D. tosaense)、細莖石斛 (D. leptoclandum)、紅鸝石斛(D. falconeri)與金草石斛(D. aurantiacum)成一群;鷹爪石斛(D. chameleon)與紅花石斛(D. miyakei)成一群;鴿石斛(D. crumenatum) 與燕石斛(D. equitans) 成一群。另外, 大雙花石斛(D. furcatopedicellatum)與小雙花石斛(D. somai)成一群,但與其它石斛蘭遺傳距離甚遠。經由DNA 分析,本研究支持原歸為石斛蘭屬的連珠石斛 (Epigeneium nakaharai)應與石斛蘭屬分開,並且也發現大雙花石斛與小雙花石斛也不適合置於石斛蘭屬內。 |
英文摘要 | The genetic relationship of 12 Dendrobium species in Taiwan was determined based on sequence analysis of the internal transcribed spacer (ITS) region of ribosomal DNA. Aligned sequences of the complete ITS region obtained from the 12 taxa and two outgroups resulted in 684 characters. Genetic distances and a phylogenetic tree were constructed, and four main clusters were generated among the 12 Dendrobium species. From the results, D. linawianum, D. moniforme, D. tosaense, D. leptocladum, and D. falconeri are grouped with D. aurantiacum; D. chameleon is grouped with D. miyakei; and D. crumenatum is grouped with D. equitans. In addition, D. furcatopedicellatum and D. somai are grouped together but are separated from other Dendrobium species. In the present study, results also support Epigeneium nakaharai not being placed in the genus Dendrobium. In addition, it may be preferable to place D. furcatopedicellatum and D. somai in a genus different from Dendrobium. |
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