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題名 | 藉由分析核糖體核酸內轉錄間隔區探討臺灣原生杜鵑種原之親緣關係=The Genetic Relationship of Rhododendron (Ericaceae) in Taiwan Based on the Sequence of Internal Transcribed Spacer of Ribosomal DNA |
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作者 | 蔡奇助; 黃柄龍; Tsai, Chi-chu; Huang, Ping-long; |
期刊 | 高雄區農業改良場研究彙報 |
出版日期 | 20041100 |
卷期 | 15:2 2004.11[民93.11] |
頁次 | 頁44-57 |
分類號 | 377.2209 |
語文 | chi |
關鍵詞 | 核糖體核酸; 內轉錄間隔區; 杜鵑花; 親緣關係; Rhododendron; Genetic relationship; rDNA; ITS; PCR; |
中文摘要 | 本研究藉由分析核糖體核酸(ribosomal DNA, rDNA) 內轉錄間隔區 (internal t ranscribed spacer, ITS)之序列, 以探討台灣20 種原生杜鵑的親緣關係。將20 種台灣原生杜鵑的ITS 序列進行比較排列後發現,在ITS1 區域有37 個變異處, 在ITS2 區域有35 個變異處。以Kimura 2-parameters 換算物種兩兩間的遺傳距離 (genet ic distance),發現這 20 種杜鵑間的遺傳距離介於0-0.062 間。經不加權平均重法 (unweighted pai r-group method analysis, UPGMA) 進行群叢分析, 並完成親緣關係樹狀圖 (phylogenet ic tree)。根據樹狀圖所呈現之物種間的親緣關係,台灣的原生杜鵑主要可以分成六群, 其中埔里杜鵑(R. lasiostylum)、紅毛杜鵑 (R. rubropilosum)、烏來杜鵑 (R. kanehirai)、南澳杜鵑(R. breviperulatum)、金毛杜鵑(R. oldhami i )、細葉杜鵑(R. noriakianum)、中原氏杜鵑 (R. nakaharai )、台灣高山杜鵑 (R. taiwanalpinum)、大屯杜鵑 (R. longiperulatum)與唐杜鵑 (R. simsi i)歸於第一群; 長卵葉馬銀花 (R. lamprophyllum)與馬銀花 (R. ovatum)歸在第二群; 南湖杜鵑 (R. hyperythrum)、森氏杜鵑 (R. morri)、台灣杜鵑 (R. formosanum) 及玉山杜鵑 (R. pseudochrysanthum) 與紅星杜鵑 (R. rubropunctatum)歸在第三群。此外, 西施花、黃花杜鵑及守城滿山紅則分別自成一群。上述ITS 序列所得的親緣關係與目前台灣原生杜鵑所歸屬的系統分類進一步比較,發現在亞屬及節的層次皆很相符。 |
英文摘要 | Genetic relationship of 20 Rhododendron species in Taiwan was derived based on the sequence analysis of the internal t ranscribed spacer(ITS) region of ribosomal DNA. Sequences of complete ITS region, including ITS1, 5.8S rDNA, and ITS2, were obtained by direct sequencing of polymerase chain reaction(PCR)amplified fragments. Gaultheria itoana was used as outgroup. Aligned sequences of ITS1 and ITS2 from 21 taxa resulted in 493 characters. According to the dendrogram, six main clusters were generated among 20 species of genus Rhododendon in Taiwan. R. hyperythrum, R. morri, R. formosanum, and R. pseudochrysanthum are grouped with R. rubropunctatum; R. lamprophyllum are grouped with R. ovatum; R. lasiostylum, R. rubropilosum, R. kanehirai, R. breviperulatum, R. oldhamii, R. noriakianum, R. nakaharai, R. taiwanalpinum, and R. longiperulatum are grouped with R. simsii. In addition, R. kawakamii, R. ellipticum and R. mariesii are three independent clusters. Molecular data from ITS sequences in the present study is in agreement with traditional systematics of Rhododendron. |
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