查詢結果分析
來源資料
相關文獻
- 超音波刺激組織工程神經導管對周邊神經再生之研究
- 染料分離、純化的應用技術
- 口咽後壁之神經鞘瘤--病例報告
- Simultaneous Quantitation of Cationic Disinfectants by High-Performance Liquid Chromatography on a Silica Gel Column Using Aqueous Eluents
- Study on the Mechanism of Enhancing Callus Formation of Fracture by Ultrasonic Stimulation and Microwave Hyperthermia
- Autotrophic Denitrification Via a Biofilm Growing on a Gas-Permeable Silicone Tube
- 不同酸性氣膠採樣器的現場測試研究
- Application of HPLC Method Using Normal Phase Column in a Comparative Pharmacokinetic Study of Two Sulpiride Tablet Formulations
- 乙二胺氣相層析分析方法之研究
- Ultrasonic Stimulation on the DP-bioglass Bone Substitute in the Rabbit Femur Condyle
頁籤選單縮合
題名 | 超音波刺激組織工程神經導管對周邊神經再生之研究=The Effects of Low-intensity Ultrasound on Peripheral Nerve Regeneration in Poly (DL-lactic Acid-co-glycolic Acid)Conduits Seeded with Schwann Cells |
---|---|
作者姓名(中文) | 龔瑞英; 廖苑晴; | 書刊名 | 銀樺學誌 |
卷期 | 3:3 民94.04 |
頁次 | 頁1-19 |
分類號 | 416.293 |
關鍵詞 | 超音波刺激; 聚乳酸-聚甘醇酸共聚合物; 矽膠管; 許旺氏細胞; Ultrasonic stimulation; Poly; DL-lactic acid-co-glycolic acid; Silicone conduit; Schwann cells; |
語文 | 中文(Chinese) |
中文摘要 | 低強度超音波,已經被證實對於神經的再生能力極具有影響力。但對於治療用低強度超音波應用於植入許旺氏細胞的神經導管的成效,目前仍舊不清楚。在這次研究中,我們試著證明,對於植入可降解性神經導管中的許旺氏細胞,超音波可以提昇其功能,進而提昇週邊神經重建的效果。將Sprague Dawley大鼠的右側坐骨神經以神經切斷術製造出一公分的缺陷,將不同材質的神經導管植入於此缺罕中。在經過一天的休息後,老鼠將接受為期兩個禮拜的超音波治療流程。超音波條件如下:頻率1MGz、強度0.2/W/cm□(SATP)、每天紿療5分鐘。而對照組則將採用相同情況的假性刺激以茲比較。6個星期後,將神經導管由大鼠體內採集出來。做組織切片分析。我們發現,採用超音脈衝攪繳的PLGA群組,不論是否植入許旺氏細胞,和對照組比較起來,神經細胞的軸索在面積和數量上均呈現顯著加速的增加。而矽膠管的實驗組上,若利用超音波刺激,將導引大量的纖維組織覆蓋在神經導管外面,相對的減緩神經軸索的再生能力。這些研究,顯示超音波刺激的技術,對於植入許旺氏細胞的PLGA神經導管的再生能力,將會是一項全新而且有效的技術。 |
英文摘要 | Low-intensity ultrasound has been proved to be effective in nerve regeneration. The relationship between therapeutic ultrasound and seeded Schwann cells within nerve conduits, however, has not been known. This study investigated the effects of low intensity ultrasound in poly(DL-lactic acid-co-glycolic acid)(PLGA)conduits seeded with Schwann cells for peripheral nerve regeneration. All conduits were implanted to 10 mn right sciatic nerve defects’ rats receiving 12 ultrasonic treatment sessions over 2 week after one day of resting. The ultrasound condition was as followed: frequency 1MHz, intensity 0.2W/cm□(SATP), treatment 5 mins/day. The sham groups were utilized under the same condition for the sake of comparison. The specimens of conduits were harvested for histological analysis after 6 weeks. Compared to the sham groups, the treatment of PLGA groups (with and without Schwann cells) with pulsed wave ultrasonic simulation resulted in a significant acceleration in number and area of regenerated axons at the mid-conduit portion of the implanted grafts. The finding was the ultrasonic stimulation on silicone groups could induce mass fibrous tissues which covered the nerve conduits and retarded the axon regeneration. These suggest that ultrasonic stimulation can be a new and effective technique for nerve regeneration on Schwann cells-seeded PLGA conduits. Nevertheless, the application of ultrasound may not be suitable for silicone conduits in nerve regeneration. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。