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題 名 | Simultaneous Determination of Bromhexine HCI and Baicalin in Chinese Compound Medicine by a Reversed-Phase Ion-Pair HPLC=逆相離子對高效液相層析法同時測定複方中藥中的鹽酸溴克辛和黃芩苷 |
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作 者 | 劉淑娟; 趙亮; 劉霞; 周峰; 蔣生祥; | 書刊名 | 藥物食品分析 |
卷 期 | 12:4 2004.12[民93.12] |
頁 次 | 頁306-310+371 |
分類號 | 412.36 |
關鍵詞 | 高效液相層析法; 藥品分析; 鹽酸溴克辛; 黃芩苷; Pharmaceutical analysis; HPLC; Bromhexine HCI; Baicalin; |
語 文 | 英文(English) |
中文摘要 | 本文首次建立了同時測定複方咳喘靈膠囊中鹽酸溪克辛 (bromhexine HCI , BHH) 和黃苓苷 (baicalin) 的逆相離子對高效液相層析法。探討了四丁基溴化銨 (TBA) 的濃度、離子對試劑的種類、三乙胺以及有機溶劑的含量對兩種分析物滯留時間的影響。選擇含有0.7mmol L-1 TBA的乙腈/水/ 磷酸/三乙胺 (22/78/0.1/0.1,v/v/v/v) 移動中目在波長225 nm分離檢測黃苓苷和BHH,結果顯示黃苓苷和BHH的波峰分別在21min和27min出現,分離度較大,峰形良好,且不受空白對照樣品的干擾。在上述優化的條件下,BHH線性範團為0.2-15.0μg mL-1,最低檢測限量是0.1μgmL-1。黃芩苷線性範園為0.1-12.0μgmL-1,最低檢測限量是0.05μg mL-1 ,平均回收率分別為97.7% (BHH) 、99.1% (黃苓苷) 。結果顯示該方法簡便、通用、快速、準確,可以作為複方中藥含此二類成分藥物的品質管制的檢驗方法。 |
英文摘要 | In this article, a reversed-phase ion-pair high-performance liquid chromatography (HPLC) method was established for simultaneous separation and quantitative analysis of bromhexine HCl (BHH) and baicalin in Chinese Ke-Chuan-Ling compound capsule. The influence of the mobile phase composition on the retention of the analytes was investigated. The optimal determination conditions consisted of acetonitrile/water/phosphoric acid/triethylamine (22/78/0.1/0.1, v/v/v/v) mobile phase with the addition of tetra-n-butylammonium bromide (TBA) (0.7 mmol L-1, terminal concentration) as pairing ion, and a detection wavelength at 225 nm. In this case, baicalin and bromhexine HCl were eluted at 21 min and 27 min respectively and were separated successfully from other ingredients. Excellent linear relationship between the peak area and concentration was obtained in the range of 0.2~15.0 mg mL-1 for bromhexine HCl and 0.1~12.0 mg mL-1 for baicalin. The limit of detection of BHH was 0.1 mg mL-1 and it was 0.05 mg mL-1 for baicalin. The average recoveries of bromhexine HCl and baicalin were 97.7% and 99.1%, respectively. From the results it can be concluded that this method is simple, versatile, and reliable for the quality control of Chinese traditional medicines containing baicalin and/or bromhexine HCl. |
本系統中英文摘要資訊取自各篇刊載內容。