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題名 | 建立骨質疏鬆症之動物模式--初報=Development of Osteoporosis Animal Models-preliminary Report |
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作者 | 陳慰宗; 施庭芳; 李碧珍; 林亞倫; 陳潤秋; 杜興洋; 璩大成; | 書刊名 | 中華放射線醫學雜誌 |
卷期 | 29:2 2004.04[民93.04] |
頁次 | 頁67-72 |
分類號 | 415.596 |
關鍵詞 | 動物模式; 骨質疏鬆症; Animal model; Osteoporosis; |
語文 | 中文(Chinese) |
中文摘要 | 背景:本研究想要藉由控制單骨質疏鬆危險因子,如切除大鼠卵巢(停經後骨質疏鬆),定期注射類固醇(類固醇引發之骨質疏鬆),及固定肢體(不動性骨質疏鬆),以期建立各類骨質疏鬆的實驗動物模式。 方法:實驗包括三種處置:(1)於9隻母鼠實施兩側卵巢切除。(2)於5隻公鼠每日腹腔注射methylpre-dnisolone sodium succinate (1ug/day)。(3)於7隻公鼠以膠火固定單側下肢。我們利用定量電腦斷層(quantitative computed tomography)配合定量假體測定皮質骨,海綿狀骨,以及二者合併測量之骨質密度,並統計實驗組與對照組間骨質密度之差異。 結果:實驗結果發現不論在皮質骨,海線狀骨,以及二者合併測量之骨質密度數值在實驗組皆較對照組低,且達到統計意義(p<0.05)。在切除大鼠卵巢(停經後骨質疏鬆)及注射類固醇動物模式中,脊椎海綿狀骨骨質密度降低的程度比皮質骨明顯(28.2% versus 4%; 28.4% versus 10%)。而在不動性骨質疏鬆動物模式中海綿狀骨與皮質骨之骨質密度佭低程度較無明顯異(10% versus 6.2%)。 結論:本研究證實了吾人可在不同條件下成功引發大鼠骨質密度減少,可為以後基礎研究與評估骨質疏鬆藥物臨床治療效果提供適當的骨質疏鬆動物模式。 |
英文摘要 | Background: The purpose of this study was to develop osteoporosis animal models by control a single risk factor for different kinds of osteoporosis, such as: to simulate postmenopausal osteoporosis by ovariectomy, to simulate steroid-induced osteoporosis by daily methylprednisolone injection, to simulate immobilization osteoporosis by fixation of unilateral hind leg. Materials and methods: We performed: (1) ovariectomy on 9 female Wistar rats, (2) daily injection of methylprednisolone sodium succinate (1ug/g/day) on 5 male Wistar rats, (3) fixation of unilateral hind leg on 7 male Wistar rats. Quantitative computer tomography in association with quantitative phantom was used to calculate the bone mineral densities of trabecular bone, cortex and the combined values in each subject. Statistical analyses were performed to compare the bone mineral densities between treatment and control groups. Results: Significant bone mineral density decrease (p < 0.05) developed in cortical bone, trabecular bone and combined measurement of both structures in treatment groups of the 3 models, as compared to their control groups. In ovariectomy and steroid-injection animal models, the extent of bone loss in trabecular bones was more prominent than in cortical bones (28.2% versus 4% and 28.4% versus 10%). In disuse animal model, the extent of bone loss in trabecular and cortical bones was similar (10% versus 6.2%). Conclusion: Our results demonstrate successful induction of bone loss by different treatments, which can be used as proper osteoporosis animal models for basic studies and evaluation of effectiveness of new drug for osteoporosis in the future. |
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