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題 名 | The Mucilage of Yam (Dioscorea Batatas Decne) Tuber Exhibited Angiotensin Converting Enzyme Inhibitory Activities=山藥黏質多醣具有血管收縮素轉化酶抑制活性 |
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作 者 | 李美賢; 林音秀; 林耀輝; 徐鳳麟; 侯文琪; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 44:4 2003.10[民92.10] |
頁 次 | 頁267-273 |
分類號 | 414.34 |
關鍵詞 | 山藥; 黏質多醣; 血管收縮素轉化酶; 混合型抑制; Angiotensin converting enzyme; ACE; HPLC; N-3-[2-furyl]acryloyl/-Phe-Gly-Gly; FAPGG; Mucilage; TLC; Yam; |
語 文 | 英文(English) |
中文摘要 | 山藥 (Dioscorea batatas Decne) 黏質多醣經由抽取與純化步驟,以 SDS 電泳膠片顯示達到均質,以 toluidine blue 染色發現其分子量大於 250kDa。以 (N-(3[2-furyl]acryloy)-Phe-Gly)(FAPGG) 為酵素反應基質 , 此一純化的山藥黏質多醣能抑制血管收縮素轉化? (102.46 到 409.84 μg/mL,分別抑制 28.7% 到 59.8% 血管收縮素轉化脢活性) 其效果隨劑量增加而增加。山藥黏質多醣對於血管收縮素轉化脢50% 抑制濃度為 256.2 μg/mL[ 對照組 captopril 7.8lnM(9.5pmole)] 。多醣類的果膠質 (102.46,204.92 和 30738μg/mL) 對血管收縮素轉化臨並沒有抑制的效果。利用螢光 silica TLC 或是逆相 C[]HPLC 檢測 FAPGG其水解產物FAP, 結果顯示山藥黏質多醣可以抑制血管收縮素轉化臨。山藥黏質多醣對於血管收縮素轉化脢為混合型抑制,而 Michaelis constant 為 0.3304mM 。 |
英文摘要 | The tuber mucilage of yam (Dioscorea batatas Decne) (YTM) was extracted and purified to homogeneity, which was confirmed by the toluidine blue staining on a sodium dodecylsulfate-polyacrylamide gel electrophoresis gel treated with 2-mercaptoethanol appearing as a single band with molecular mass larger than 250 kDa. This purified YTM was shown by spectrophotometric method to inhibit angiotensin converting enzyme (ACE) in a dose-dependent manner (28.7 to 59.8% ACE inhibition, respectively, by 102.46 to 409.84 μg/mL YTM) using (N-(3-[2-furyl] acryloyl)-Phe-Gly-Gly) (FAPGG) as a substrate. The concentration of YTM required for 50% inhibition (IC..) of ACE activity was 256.2 μg/mL while that of captopril was 0.00781 uM (0.0095 nmole). The commercial polysaccharide pectin (102.46 to 307.38 ug/mL) showed no inhibitory activity against ACE. Using fluorescent silica TLC or C18 reverse phase HPLC to detect FAPGG and FAP, the results also showed that YTM inhibited ACE. The YTM showed mixed type inhibition against ACE, and the Michaelis constant in the presence of YTM was 0.33 mM. Consumption of yam tubers may benefit people's health. |
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