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題名 | Simultaneous Determination of Albendazole, Thiabendazole, Mebendazole and Their Metabolites in Livestock by High Performance Liquid Chromatography=畜產品中動物用藥Albendazole、Thiabendazole、Mebendazole及其代謝產物檢驗方法之建立 |
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作者 | 蘇淑珠; 張津琳; 張碧秋; 周薰修; | 書刊名 | 藥物食品分析 |
卷期 | 11:4 2003.12[民92.12] |
頁次 | 頁307-319+355 |
分類號 | 412.37 |
關鍵詞 | 動物用藥; 高效液相層析法; Veterinary drug; Albendazole; Thiabendazole; Mebendazole; High performance liquid chromatography; |
語文 | 英文(English) |
中文摘要 | 本研究探討微透析聯結逆相高效能液相層析直接分析茶品中之兒茶素。茶樣品中之兒茶素擴散進入中空透析膜中之灌注液中,直接注射進入高效能液相層儀進行分析。C8層析管柱以乙腈/pH4.0磷酸緩衝水溶液進行梯度沖提分離兒茶素,以UV diode-array偵測。研究探討影響透析效能之因素,包括灌注液與樣品溶液之條件,並且提出一個分析茶及茶品中之兒茶最成分的簡單快速方法。利用所提出之方法分析茶及茶品中之epicatechin、epicatechingallate、epigallocatechin和epigallocatechin gallate。 |
英文摘要 | An analytical method using high performance liquid chromatography (HPLC) was developed for the simultaneous determination of thiabendazole, 5-hydroxythiabendazole, albendazole sulfoxide, albendazole sulfone, albendazole 2-aminosulfone and mebendazole in livestock. These six benzimidazoles were extracted with acetonitrile under basic conditions, followed by a Sep-Pak C18 cartridge cleaning procedure. The HPLC separation was achieved on a Cosmosil 5C18-MS-II column (5 µm, 4.6 mm i.d. ×250 mm) using gradient elution with acetonitrile and 0.02M sodium dihydrogen phosphate (pH 3.3), and detection was performed with photodiode array and fluorescence detector. Good linearity was observed from the calibration plot at concentrations from 0.05 to 2.50 µg/mL. Recovery studies of the analytes were performed at 0.02, 0.10 and 1.00 ppm spiked levels. The average recovery of 5-hydroxythiabendazole and mebendazole at low concentrations ranged from 71.4 to 87.1% and ranged from 81.6 to 104.3% at other concentrations, with coefficient of variation less than 5.9%. The detection limit for benzimidazoles was between 0.005~0.030 ppm under photodiode array detection and 0.004~0.020 ppm under fluorescence detection. The coefficient of variation of intra-day and inter-day assays was lower than 1.92% and 7.22%, respectively. These results indicated that the developed method had an acceptable precision. This method was applied to detect benzimidazoles in samples of pork, swine liver, beef, lamb, bovine milk and goat milk purchased from various markets in Taipei. The results showed that none of the six benzimidazoles were detected in the thirty samples. |
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