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題名 | Age-Related Changes in Osteogenic Potential of Bone Marrow Stem Cells=年齡增長後骨髓幹細胞骨生成能力改變情形之研究 |
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作者 | 史中; 夏毅然; | 書刊名 | 中華民國口腔顎面外科學會雜誌 |
卷期 | 13:2 2002.09[民91.09] |
頁次 | 頁171-177 |
分類號 | 398.1 |
關鍵詞 | 鹼性磷酸酵素; 骨群體; 骨生成能力; 幹細胞; Alkaline phosphatase activity; Bone colony; Osteogenic potential; Stem cell; |
語文 | 英文(English) |
中文摘要 | 年齡增長後骨吸收增加和骨生成或少,可誘發老年性骨質明顯減少和增加骨折的發生。但年齡增長後骨生成明顯的減少是否與骨髓間黨 (mesenchymal) 或骨幹細胞 (osteogenic stem cells) 之骨生成能力的改變有密切關聯,並未定論。本研究旨在探討老年與成年小白鼠骨髓間葉或骨幹細胞骨生成能力是否有明顯差異。方法:使用小白鼠骨髓細胞體外骨生成模式和比重梯度分離法,分別自2及20個月BALB/c種雄性小白鼠獲得骨幹細胞 (bone stem cell),然後將6×10 6小比重骨髓細胞加入培養且中飼養層土培養。l4天後,使用von kossa染色法、光源鏡檢、酵素組織化學和第一型膠原蛋白合成法等方法分析。 結果發現在鼠齡為20月之小白鼠其骨質密度顯著降低,並且小比重骨髓細胞的培養所形成骨群體的數目、第一型膠原蛋白合成能力及鹼性磷酸酵素活性與2月組比較,均有顯著減少的趨勢 (p<0.01) ,並且礦物化作用的進行也呈現延遲的情形。本研究結果顯示骨髓中骨幹細胞數目的減少及其骨生成能力的降低可能是導致年齡增長後骨質生成減少或骨質疏鬆的主要原因之一。 |
英文摘要 | An increase in bone resorption and a decrease in bone formation with age could result in age-related osteopenia and the occurrence of fractures. However, the correlation between changes in osteogenic potential of bone marrow mesenchymal or osteogenic stem cell and age-related osteopenia is still unclear. The purpose of this study was to investigate the difference of osteogenic potential of bone marrow stem cells between adult and aged mice. With density gradient separation method, bone stem cells were harvested from 2- and 20-month-old BALB/ c mouse. Six million light density bone marrow cells were plated on feeder layer in BGJb medium for 14 days. The changes in osteogenic potential was analyzed using von Kossa stain, alkaline phosphatase assay, type I collagen synthesis assay, and light microscopy. Fourteen days after culture, number of colonies, phosphatase expression and type I collagen in 20-month-old mice were significantly decrease as compared to the control (2 month) group (p < 0.01). In addition, significant decrease in BMD in was noted 20-month-old mice group as compared to the controls. The results of this study indicated that age-related osteopenia could be resulted from the decrease in bone marrow stem cells and osteogenic potential. |
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