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頁籤選單縮合
題名 | Establishment of a Suitable in Vitro Cell Culture System for the Study of Osteogenesis=適合研究骨生成之細胞培養模式的建立 |
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作者 | 史中; 夏毅然; | 書刊名 | 中華民國口腔顎面外科學會雜誌 |
卷期 | 12:2 2001.09[民90.09] |
頁次 | 頁73-80 |
分類號 | 398.1 |
關鍵詞 | 鹼性磷酸酵素; 骨群體; 間葉細胞; 礦物化過程; Alkaline phosphatase; Bone colony; Mesenchymal cells; Mineralization; |
語文 | 英文(English) |
中文摘要 | 本研究旨在探討自未曾發生骨生成之胎鼠頭蓋骨組織分離之單一細胞是否可進行增生、分化和形成礦物化骨組織。13天大SD胎鼠額骨和頂骨區域之頭蓋骨組織經由膠原蛋白酵素處理,獲得之單一細胞以BGJb培養液進行培養。結果發現:培養12天時,未礦物化群體形成並且開始礦物化。骨群體形成的數目和植入的細胞存在正相關關係。光源鏡檢可見礦物化骨基質表面有造骨細胞貼附。另外,隨著植入細胞數目的增加,鹼性磷酸酵素活性和第一型膠原蛋白合成均有明顯增加的情形。本研究首次證實自未曾發生骨生成之胎鼠頭蓋骨組織分離之單一細胞可增生、分化和形成礦物化骨基質。 |
英文摘要 | The purpose of this study was to investigate whether cells isolated from fetal rat calvariae without pre-existent osteogenesis still retain their ability to proliferate, differentiate, and form mineralized bone. Mesenchymal cells were isolated from 13-day fetal Sprague-Dawley rat calvaria of frontal and temporal regions by collagenase digestion and cultured in BGJb medium. After 12 days in culture, unmineralized colonies were formed and began to mineralize with addition of 10 mM βglycerophosphate. There was linear correlation between the number of calvarial cells plated and the number of bone colonies formed. Histologic sections revealed that colony consisted of a calcified matrix with elongate or cuboid osteoblasts covering the surface. In addition, significant increase in both alkaline phosphatase activity and type I collagen synthesis were positively correlated to the cell plated. This study first demonstrated that cells enzymatically isolated from 12-13-day fetal rat calvariae without preexistent bone formation show various phenotypes including formation of mineralized bone matrix in culture. This culture system was reproducible and could be used for studying the regulation of bone differentiation and mineralization. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。