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題 名 | Detection of Genetically Modified Soybeans by PCR Method and Immunoassay Kits=以PCR方法與市售免疫套組檢測基因改造大豆 |
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作 者 | 林旭陽; 江靜雯; 施養志; | 書刊名 | 藥物食品分析 |
卷 期 | 9:3 2001.09[民90.09] |
頁 次 | 頁160-166 |
分類號 | 412.37 |
關鍵詞 | 免疫檢測套組; 基因改造大豆; GM soybeans; PCR; Immunoassay; |
語 文 | 英文(English) |
中文摘要 | 以PCR方法與市售免疫套組探討鑑別檢測基因改造大豆之可行性。針對Roundup Ready�藎穧]改造大豆(RRS,Monsanto公司)殖入基因與品種特性基因選定不同引子,進行PCR與ELISA方法檢測。用以鑑別基因改造大豆之引子共四對,分別為35S(針對35S-promoter,源自cauliflower mosaic virus)、NOS(nopaline synthase-terminator,源自Agrobacterium tumefacients)、EPSPS(5-enolpyruvylshikimate-3-phosphatesynthase,源自A. tumefaciens strain CP4)及LE(品種特性基因lectin)設計檢測。結果顯示,大豆檢體以35S及EPSPS引子檢測時,其最低檢測量均為0.1%(w╱w),NOS 則為 1 %(w╱w);檢體並以LE引子-PCR反應確定均為大豆產品。經以不同免疫檢測套組試驗,條片法較適用於定性之檢測,易於操作、攜帶和判讀;而ELISA方法除可鑑別基因改造大豆外,亦適用於含量在0-2.5%之檢體定量檢測。以市售免疫檢測套組檢測的結果與PCR法檢測的結果相符,均可區分鑑別一般大豆與RRS基因改造大豆。 |
英文摘要 | The suitability of detecting genetically modified (GM) soybeans by polymerase chain reaction (PCR) and immunoassay kits is determined. Primers specific for inserted genes in the Roundup Ready�� soybean (RRS, Monsanto company) were applied. Four pairs of primers, namely, 35S (35S-promoter, originated from cauliflower mosaic virus), NOS (nopaline synthase-terminator, derived from Agrobacterium tumefaciens), EPSPS (5-enolpyruvylshikimate-3-phosphate synthase, obtained from A. tumefaciens strain CP4) and LE (endogenous gene lectin) were used to identify the GM soybeans. The detection limit of PCR was 0.1% (w/w) GM soybeans when primers 35S and EPSPS were used, and 1% when primers NOS were used. All soybean samples wer evidenced by LE primer-PCR as soybean products. Results of Immunoassays by two kinds of kits, strip and ELISA were matched with PCR's and feasibility of quantitatio detecting GM soybeans is determined among 0∼2.5%. The data further revealed that the PCR method and immunoassay kits can sufficiently differentiate GM soybeans from non-GM products. |
本系統中英文摘要資訊取自各篇刊載內容。