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題名 | 豬胸腺萃取物之細胞活性測試=Cell Proliferation Assay of Porcine Thymus Extract |
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作者 | 賴坤男; | 書刊名 | 臺灣糖業公司研究所研究彙報 |
卷期 | 171 2001.03[民90.03] |
頁次 | 頁39-46 |
分類號 | 437.653 |
關鍵詞 | 豬胸腺萃取物; 脾臟細胞; 巨噬細胞; 皮膚纖維母細胞; 細胞分裂活性測試; Porcine thymus extract; Spleen cell; Macrophage; Skin fibroblast; Cell proliferation assay; |
語文 | 中文(Chinese) |
中文摘要 | 為了瞭解改良萃取法所得豬胸腺萃取物其相關細胞分裂活性能力,針對3種細胞,包括老鼠脾臟細胞、巨噬細胞及人類皮膚纖維母細胞所作的XTT細胞分裂活性測試,顯示豬胸腺萃取物具有活化非特定性及特定性免疫細胞以及皮膚細胞之功效,而其活化機制可能來自於多重而非單一的因素。 |
英文摘要 | In order to understand the effects of porcine thymus extract prepared by a previously developed extraction method on the proliferation of related cells, three cell types--mouse spleen cell, mouse macrophage, as well as human skin fibroblasts--were cultured to assay the cell proliferation effects of the extract. XTT cell proliferation assay clearly shows that porcine thymus extract not only activates immunologic cells in nonspecific (mouse spleen cell) and specific (mouse macrophage) way but also activates human skin fibroblast. Further studies may show that the cell activation effects of porcine thymus extract result from multiple rather than a single factor. |
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