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頁籤選單縮合
題 名 | Diagnosis of Pemphigus Vulgaris by Immunoblotting of Cultured Human Keratinocyte Cell Extracts=以培養之人類角質細胞萃取物進行免疫墨點法診斷尋常性天疱瘡 |
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作 者 | 陳柏宏; 陳秋月; 許晴哲; | 書刊名 | 中華皮膚科醫學雜誌 |
卷 期 | 19:2 2001.06[民90.06] |
頁 次 | 頁93-97 |
分類號 | 415.765 |
關鍵詞 | 免疫墨點法; 尋常性天疱瘡; 直接免疫螢光法; 間接免疫螢光法; 自體免疫水疱症; Autoimmune bullous diseases; Pemphigus vulgaris; Immunoblotting; Direct immunofluorescence; Indirect immunofluorescence; |
語 文 | 英文(English) |
中文摘要 | 尋常性天疱瘡是自體免疫水疱症中主要的一種疾病。而自體免疫水疱症包括尋常性天疱瘡,類天疱疹,後天性表皮裂解性水疱症,線狀IgA皮膚症及其他新的水疱疾病等。尋常性天疱瘡的診斷主要是依據臨床,組織病理及免疫學上之標準。而免疫墨點法則是一新發展的方法,用以偵測這些自體免疫水疱症的特定抗原。在此研究中,我們成功的在五位尋常性天疱瘡患者以培養之人類角質細胞培養萃取物進行免疫墨點法偵測到antidesmogleins之自體抗體。其中四位患者的血清中可偵測到分子量130 kDa的Dsg3,而另一位患者則是可同時偵測到130 kDa與160 kDa兩種蛋白質。吾人建議免疫點墨法應與直接免疫螢光,間接免疫螢光法一起使用,以求得對自體免疫水疱症能有更好的診斷與分類。 |
英文摘要 | Pemphigus vulgaris is one of the major autoimmune bullous diseases which include pemphigus vulgaris, bullous pemphigoid, epidermolysis bullosa acquisita, linear IgA dermatosis, and many more new entities. The diagnosis of pemphigus vulgaris depends on clinical, histological and immunological criteria. Immunoblotting is a newly developed technique used to detect the specific antigens of these autoimmune bullous diseases. In this study, we successfully detected the anti-desmogleins autoantibodies from sera of five well-documented pemphigus vulgaris patients by using immunoblotting of cultured human keratinocyte cell extracts. Sera from four of these patients reacted with the 130 kDa desmoglein (Dsg) 3 protein band, one reacted with both 130 kDa and 160 kDa protein bands. We recommend immunoblotting should be used together with direct immunofluorescence and indirect immunofluorescence for the better diagnosis and classification of autoimmune bullous diseases. |
本系統中英文摘要資訊取自各篇刊載內容。