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頁籤選單縮合
題 名 | 利用真核系統表現豬第二型環狀病毒ORF 2 蛋白=Expression of the Recombinant ORF 2 Protein of Porcine Circovirus Type 2 in Eukaryotic System |
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作 者 | 王羣; 邱芮瑜; 黃天祥; 鍾明華; 趙磐華; 郭應誠; | 書刊名 | 行政院農業委員會家畜衛生試驗所研究報告 |
卷 期 | 42 2007.12[民96.12] |
頁 次 | 頁79-88 |
分類號 | 437.657 |
關鍵詞 | 豬環狀病毒; 間接免疫螢光法; 聚丙烯醯胺膠片; 西方轉印法; Porcine circovirus type 2; Indirect immunofluorescense assay; SDS-PAGE; Western blotting; |
語 文 | 中文(Chinese) |
中文摘要 | 首先萃取臺灣豬第二型環狀病毒(porcine circovirus type 2; PCV2)分離株之病毒核酸,然後利用聚合酶鏈反應(polymerase chain reaction, PCR )增幅豬第二型環狀病毒之第二開毒窗(open reading frame 2; ORF2)基因全長片段。再將該基因片段植入表現載體中(pCI-neo),經篩選後,選擇嵌有該基因之重組質體,並將此重組質體轉染(transfect)至真核細胞(PK-15 cell line)內進行重組蛋白表現,並以間接免疫螢光法(indirect immunofluorescence assay, IFA)、聚丙烯醯胺膠片(SDS-PAGE)以及西方轉印法(western blotting)進行分析,可觀察到重組蛋白在真核細胞成功的表現出來。 |
英文摘要 | Porcine circovirus type 2 represents the causative agent of a new porcine disease which spread all over the world during the past several years. In this study, viral DNA of a Taiwanese PCV2 strain was extracted and its open reading frame 2 (ORF2) gene was amplified by PCR. The PCR product was purified and then cloned into the pCI-neo vectors. The recombinant vector was transfected into PK-15 cells. The recombinant protein was expressed successfully in PK-15 cells, which could be detected by using indirect immunofluorescence assay and Western blotting analysis. |
本系統中英文摘要資訊取自各篇刊載內容。