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題 名 | Diverse Effects of Ba[fec7] and Co[fec7] on Fura-2 Fluorescence Signals Induced by ATP in Cultured Bovine Aortic Endothelial Cells=鋇、鈷離子對腺苷三磷酸引發培養牛主動脈內皮細胞內Fura-2螢光訊息不同作用之研究 |
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作 者 | 饒錦基; 蔡麗敏; 楊生湳; 吳劍男; 楊忠謀; | 書刊名 | 醫學研究 |
卷 期 | 21:1 2001.02[民90.02] |
頁 次 | 頁9-19 |
分類號 | 360 |
關鍵詞 | 內皮細胞; 鈷離子; 鋇離子; 螢光訊息; 鈣離子波動; Fura-2; Ba[fec7]; Co[fec7]; Ca[fec7] oscillation; Endothelial cells; |
語 文 | 英文(English) |
中文摘要 | Fura-2螢光訊息(F(subscript 350)/F(subscript 380))是用來偵測細胞內鈣離子濃度變化的最佳利器。在含鈣離子溶液中,腺苷三磷酸(ATP)會激發培養牛主動脈內皮細胞螢光訊息增加並發生波動的現象。鈷離子能抑制ATP引發的螢光訊息增加;鋇離子則否。在無鈣離子的溶液中,ATP(3 µM)僅引發一短暫單一訊息;此訊息變化的最大增加速率為0.22±0.03 ratio/秒,恢復時間常數為17.7±0.08秒,面積為23.3±2.1 ratio.秒(n=16)。鋇離子(2 mM)在無鈣離子的溶液中,會增加內皮細胞螢光訊息,從0.82±0.01增加到0.92±0.02(p<0.05, n=31);隨後加入3 µM ATP引發一獨特的短暫單一訊息,此訊息變化的最大增加速率為0.42±0.03 ratio/秒(p<0.05),在短暫下降後螢光訊息持續維持在較高的2.16±0.04比值單位(p<0.05)。當細胞外鈣離子被鈷離子所取代,ATP(3 µM)引發短暫螢光訊息波動具有較長的恢復時間常數39.4±2.3秒(p<0.05, n=23),最大增加速率為0.18±0.02 ratio/秒,與對照組比較並無統計上之差別意義。在檢視單一螢光波長(F(subscript 350))強度時發現,鈷離子能抑制ATP引發的螢光強度增加;鋇離子不但增加單一螢光強度,同時增加螢光訊息比值(F(subscript 350)/F(subscript 380)),特別是在無鈣離子的狀況下。本研究結果顯示,在培養牛主動脈內皮細胞ATP激發細胞內鈣離子的釋放,但是要維持細胞內高濃度的鈣離子波動,在細胞外必須要有鈣離子的存在。鋇離子能通透細胞膜,並與鈣離子競爭結合螢光染料發光。鈷離子能抑制鈣離子流通細胞膜,但對受器調控細胞內訊息傳遞所引發鈣離子的波動則無影響。 |
英文摘要 | Diverse effects of Ba²+ and Co²+ on fura-2 fluorescence ratio signals (FR, F(subscript 350)/F(subscript 380) were examined in cultured bovine aortic endothelial cells (BAECs). ATP (3 µM) induced FR oscillations in normal Ca²+-containing solutions. Co²+, but not Ba²+, inhibited ATP-induced FR oscillations. In Ca²+-free solutions, 3 µM ATP only elicited a single FR transient. When Ba²+ replaced Ca²+ in the perfusate, 3 µM ATP provoked a distinct FR transient. The maximal rate of rise of the signal was greater than seen without Ba²+ and the FR increased and maintained to a high steady level. When external Ca²+ was replaced by Co²+, ATP-induced FR transient oscillated with a long recovery time constant. In a survey of the single fluorescence at excitation wave-length 350 nm (F(subscript350)), Co²+ decreased the intensity in the presence of ATP and Ca²+. Ba²+ however, increased F(subscript350) and FR as well, especially in Ca²+-free solutions. The results suggest that ATP-induced Ca²+ fluctuation is balanced by Ca²+ release from internal stores and influx from external Ca²+. Ba²+ seems to be permeable through Ca²+ channels and competes with Ca²+ for the dye. Co²+ blocks Ca²+ channels but does not inhibit ATP-mediated Ca²+ release in cells. |
本系統中英文摘要資訊取自各篇刊載內容。