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題 名 | In Vitro and in Vivo Photosensitizing Capabilities of 5-ALA in Vascular Endothelial Cells=5-ALA光顯劑於血管內皮細胞試管內及試管外光顯能力之評估 |
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作 者 | 張承仁; 馬淑芳; 魏福全; | 書刊名 | 長庚醫學 |
卷 期 | 22:2 1999.06[民88.06] |
頁 次 | 頁181-188 |
分類號 | 416.36 |
關鍵詞 | 光顯劑; 光照療法; 顯微內皮細胞; Photosensitizer; Photodynamic therapy; Microvascular endothelial cells; |
語 文 | 英文(English) |
中文摘要 | 背景:本實驗之目的在探討利用合適之光顯劑,配合光照療法來治療一些複雜性 的血管瘤。 而運用試管內及試管外模型來評估 5-aminolevulinic acid (5-ALA) 之光顯能 力則是我們的目的之一。 方法:試管內光顯能力之評估可檢視在顯微內皮細胞內 5-ALA 被 吸收後之位置。 而細胞經光照療法後死亡與存活比例可以較所用 5-ALA 之各種濃度及波長 630 塵米之雷射光照射後而得知。同時,亦可運用此法於�臚妍坁姨珓活A並測量�蘋a在經光 照療法後遭受破壞的程度。 結果:顯微內皮細胞於 5-ALA 濃度 35 微克 / 毫升時, 接受 630 塵米雷射光,強度 100 毫瓦 / 平方公分之照射,顯示約百分之五十細胞的致死量。而 動物模型中, �覺筐� 200 毫克 / 公斤之 5-ALA 在 630 塵米雷射光,強度 80 毫瓦 / 平 方公分的照射下其傷害深度為 362.5 ± 27.6 微米。而強度 100 或 120 毫克 / 平方公分 時,其破壞深度各為 732.5 ± 29.14 及 792.5 ± 36.0 微米。 結論:5-ALA 有足夠的能 力破壞人類之顯微內皮細胞。然於體內被各種不同組織吸收破壞之程度及時間相互影響之關 係則是吾人日後應進一步探討的課題。 |
英文摘要 | Background:The object of our study is to evaluate the feasibility of photodynamic therapy for complicated hemangiomas. We studied the effect of the photosensitizing agent 5-aminolevulinic acid (5-ALA) in both in vitro and in vivo models. Methods:The in vitro photosensitizing activity of 5-ALA was examined in a microvascular endothelial cell (MEC) culture system. 5-ALA was added in various concentrations and the cells were illuminated at 630 nm. The precentage of MEC killed was measured by either a Live/Dead assay or an lactate dehydrogenase (LDH) assay. The effect of varying the light energy dose develivered at 630 nm after the adminstration of 5-ALA was studied by determining the amount of necrosis produced in chicken combs. Results:The comination of 5-ALA at a concentration of 35 ug/ml, and illumination by 100 m W/cm �� of laser light at 630 nm wavelength, caused 50% cell kill in the MEC culture system. Chicken combs of animals that received 200 mg/kg of 5-ALA and illumination at a power density of 80 m W/cm �� had a depth of injury of 362.5 ± 27.6 um upon histological examination. Those combs that received 100 or 120 m W/cm �� showed a depth of injury of 732.5 ± 29.1 and 792.5 ± 36.0 um respectively. Conclusion:5-ALA is effective in sensitizing human MEC to laser illumination. However, the degree of absorption and tissue destruction in different anatomical structures should be considered in future clinical studies. |
本系統中英文摘要資訊取自各篇刊載內容。