查詢結果分析
相關文獻
- 以體外培養方法探討牛隻濾泡細胞分化成黃體細胞的機制
- 生長分化因子之生物功能
- 山羊用體內助孕素釋放器之製作與應用
- Alkali Syenites and Associated Rocks in Chintan, Northern Taiwan: A Reappraisal
- 進行性指掌角化症表皮最終分化及角質層功能異常之探討
- 花生花藥培養的研究(1)--不同物種花生花藥培養癒合組織誘導及芽體分化的探討
- 玉屏風散單味藥及不同組合方劑在體外試驗對單核細胞釋放第2介白質及前列腺素E[feaf]之影響
- 腎氣丸對塵蟍激發喘天竺鼠呼吸道發炎與免疫之影響
- 琥珀散治療原發性痛經之研究
- Embryonic Cell Lines Derived from Morula to Late Hatched Blastocyst Stages in Pigs
頁籤選單縮合
題名 | 以體外培養方法探討牛隻濾泡細胞分化成黃體細胞的機制=Regulation of in Vitro Differentiation of Bovine Follicular Cells into Luteal Cells |
---|---|
作者姓名(中文) | 蕭宗法; | 書刊名 | 中國畜牧學會會誌 |
卷期 | 29:3 2000.09[民89.09] |
頁次 | 頁219-228 |
分類號 | 437.21 |
關鍵詞 | 助孕素; 顆粒性細胞; 包膜細胞; 分化; 前列腺素; Prostaglandin F[feaf]扵; Forskolin; Progesterone; Granulosa cell; Theca cell; Differentiation; |
語文 | 中文(Chinese) |
中文摘要 | 牛隻動情週期中,卵巢濾泡類固醇分泌細胞(顆粒性與包膜細胞,granulosa and theca cells)分化成黃體類固醇分泌細胞(大黃體與小黃體細胞,large and small luteal cells)的機制,至今仍不清楚。本次試驗自雌性素活化過的濾泡中,分離出顆粒性與包膜細胞為試驗材料。試驗一比較forskolin(cAMP路徑)、高密度脂蛋白(high density lipoprotein, HDL,提供膽固醇基質)及胰島素(insulin)對細胞合成助孕素(P4)的影響。體外培養顆粒性細胞最初九天內,僅胰島素可刺激P4的合成(為對照組的3.4倍),而HDL與forskolin則否,但三種試劑同時存在可以顯著促進P4的合成(20倍);試劑 forskolin可刺激包膜細胞合成P4(2.9倍),但HDL與胰島素則未見類似的效果,三種試劑同時存在也顯著促進P4的合成(65倍)。試驗二以含有HDL及胰島素之培養液培養細胞,探討 forskolin在建立細胞對PGF2α反應的影響,添加forskolin可促進顆粒性與包膜細胞分泌P4的功能,但PGF2α並未明顯改變細胞的P4分泌量。經forskolin處理的顆粒性細胞,PGF2α有增加其細胞數及降低單位細胞P4分泌的趨勢。綜合試驗結果推論,胰島素、HDL與 forskolin可活化不同路徑,來促進濾泡細胞分化成黃體細胞,三者皆為分化過程所必需。顆粒性細胞雖然對PGF2α沒有反應,但其它細胞形態特徵與大黃體細胞類似。 |
英文摘要 | The steroidogenic cells of the ovarian follicle (granulosa and theca cells) differentiate into the steroidogenic cells of the corpus luteum (large and small luteal cells) by mechanisms that remain undefined. In this study we isolated pure populations of granulosa and theca cells from estrogen-active bovine follicles. In Experiment 1 we evaluated the effects of three agents that act by distinct mechanisms to stimulate luteal steroidogenesis: forskolin (cAMP pathway), lipoprotein (provide cholesterol substrate) and insulin on cellular production of progesterone(P4). During the first nine d of culture, P4 production by granulosa cells was stimulated by insulin(3.4X control) but not by forskolin or lipoprotein alone and the three agents combined stimulated a 20-fold increase in P4. In theca cells P4 was stimulated by forskolin (2.9X) but not by lipoprotein or insulin and the three agents combined stimulated a 65-fold increase in P4 . In Experiment 2, we cultured granulosa and theca cells with insulin and HDL and evaluated the role of forskolin in the establishment of prostaglandin F2α ( PGF2α) responsiveness. Forskolin stimulated an increase in P4 production by both granulosa and theca cells. Neither cell type had a significant change in P4 in response to PGF2α .There was a tendency for PGF2αto increase cell number and to decrease P4 production (on a per cell basis) in forskolin treated granulosa cells. In conclusion, insulin, lipoprotein and forskolin appear to activate distinct stimulatory pathways that are all required for differentiation of follicular cells into luteal cells. The granulosa cells acquire properties similar to large luteal cells although it is not yet clear if they acquire PGF2α responsiveness. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。