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題 名 | Study of Propofol in Bovine Aortic Endothelium:I.Inhibitory Effect on Bradyhinin-Induced Intracellular Calcium Immobilization=Propofol 於牛主動脈內皮細胞之研究:1.對Bradykinin引起細胞內鈣離子移動之抑制現象 |
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作 者 | 戴裕庭; 吳啟誠; 吳鋼治; 張懷嘉; 陳廷貴; 陳瑞明; 陳大樑; | 書刊名 | 麻醉學雜誌 |
卷 期 | 38:4 2000.12[民89.12] |
頁 次 | 頁181-186 |
分類號 | 416.5 |
關鍵詞 | 內皮細胞; 鈣; Propofol; Endothelium; Calcium; Bradykinin; |
語 文 | 英文(English) |
中文摘要 | 背景:臨床上Propofol引起之血壓下降與心跳變慢,推論與細胞內鈣离子濃度變化有關。本實驗以牛主動脈內皮細胞模式,探討propofol對於bradykinin引起之細胞內鈣離子移動之影響,以推論其臨床現象可能之機轉。方法:本研究以培養之牛主動脈內皮細胞株(Gm7372a)為對象,先以螢光染劑Fluo-3處理細胞,再與0.01mM之Propofol於37℃下共存30分鍾後,加入bradykinin,於螢光色層光譜儀(共焦顯微鏡)下,顴察bradykinin刺激下,內皮細胞內鈣離子自細胞器質中釋放出來,形成遊離鈣離子之明亮「熱點」產生之情形,與對照組比較,并以各個細胞內十個不同區域之不同時間動態變化,測量細胞內鈣移動情形,受propofol存在影響之程度。結果:加入bodykinin之後,數秒內即於細胞質與核內產生極度強烈之「熱點」,顯示遊離鈣離子自細胞內器質大量釋放,并於3分鍾內逐漸減弱其強度。如先以0.01mM propofol處理後,bradykinin刺激產生之亮點,無論在強度及持續時間上,均大量降低。以時間為構軸顴察之,加入Propofol後,於細胞內不同區域中,其遊離鈣離子顯象亮點,皆明顯減少(P<0.05),且亮點維持時間亦縮短,統計上有明顯之差別。結論:本實驗顯示內皮細胞在propofol之存在下,可以明顯地抑制因bradykinin刺激所誘導細胞內鈣之移動,進而可說明propofol造成血管舒張,可能機轉與其抑制細胞內鈣移動有關。 |
英文摘要 | Background: Propofol has been found to affect the intracellular calcium concentration with clinical manifestations of hypotension and bradycardia. The purpose of this study is to examine the effect of propofol on intracellular calcium immobilization in bovine aortic endothelium under the stimulation of bradykinin. Methods: In order to validate the effect of propofol on the alteration of intracellular calcium concentration, we used the cultured bovine endothelial cells (Gm 7372a) to measure the calcium immobilization within the cells preincubated with or without propofol of clinical concentration. Using Fluo-3 staining and a fluorescence spectrophotometer (confocal microscope), intracellular calcium immobilization was demonstrated by the appearance of "hot spots" within the cytoplasm and perinuclear regions after addition of bradykinin to the cells. The changes of fluorescence density measured within these areas versus the effect of time were analyzed and compared with the cells in control group. Results: After addition of bradykinin, intracellular calcium hot spots increased dramatically within seconds and reached a maximal level within 20 seconds. The concentrations of calcium gradually decreased to a constant level after about 3 mm following the addition of bradykinin to the cells. With pretreatment of propofol at 0.01 mM and 37°C for 30 mm, the immobilization of intracellular calcium from the intracellular stores were significantly inhibited that was demonstrated by the decreased appearance of hot spots when compared with control. Conclusions: Our data demonstrated that under the stimulation of bradykinin, propofol at 0.01 mM, could inhibit intracellular calcium release from the intracellular stores in bovine aortic endothelial cells. This phenomenon might explain the possible mechanism for the clinical manifestations of hypotension and/or bradycardia associated with propofol. |
本系統中英文摘要資訊取自各篇刊載內容。