查詢結果分析
相關文獻
- Effects of Antiglaucoma Drugs on Calcium Mobility in Cultured Corneal Endothelial Cells
- Effects of Antiglaucoma Drugs and Corticosteroids on the Cellular Glucose Uptakein Cultured Porcine Corneal Endothelial Cells
- Cellular Cytotoxicity of Antiglaucoma Drugs in Cultured Corneal Endothelial Cells
- The Outcomes of Trabeculectomy with or Without Mitomycin in ICE Syndrome
- Endothelial Cell Loss Induced by Phacoemulsification Occurs Through Apoptosis
- 角膜內皮細胞移植
- 角膜內皮細胞移植
- Alteration of Glucose Uptake in Cultured Human Corneal Endothelial Cells Grown in High Glucose Media Via cAMP-Dependent Pathway
- 長期使用局部抗青光眼藥物對結膜之影響
- 硬式透氣隱形眼鏡的配戴對於角膜內皮細胞的影響
頁籤選單縮合
題名 | Effects of Antiglaucoma Drugs on Calcium Mobility in Cultured Corneal Endothelial Cells=抗青光眼藥對角膜內皮細胞內鈣離子流動性的影響 |
---|---|
作者 | 吳國揚; 洪秀貞; 王惠珠; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷期 | 22:2 民95.02 |
頁次 | 頁60-67 |
分類號 | 418.2323 |
關鍵詞 | 抗青光眼藥; 鈣離子流動性; 角膜內皮細胞; Antiglaucome drugs; Calcium mobility; Corneal endothelial cells; |
語文 | 英文(English) |
中文摘要 | 本實驗主要是探討不同的抗青光眼藥,包括 betaxolol,timolol,levobunolol,brimonidine,carteolol,dipivefrin,dorzolamide,brinzolamide,latanoprost,unoprostone,pilocarpine 對牛角膜內皮細胞內鈣離子流動性的影響。不同的青光眼藥由原液稀釋成 1/100,1/1,000 及 1/10,000 三種濃度。細胞經 fura-2-AM 吸收後以螢光光度計測量細胞內鈣離子濃度的變化。結果發現 timolol (58 µM、5.8 µM),levobunolol (171 µM、17.1 µM 及 1.71 µM), betaxolol (162 µM、16.2 µM 及 1.62 µM),carteolol (680 µM 及 68 µM),dipivefrin (28 µM 及 2.8 µM),dorzolamide (616 µM 及 61.6 µM),brinzolamide (260 µM),latanoprost (1.1 µM),unoprostone (28.2 µM、2.82 µM 及 0.282 µM) 以及 pilocarpine (408 µM、40.8 µM) 都會明顯增加角膜內皮細胞內鈣離子的濃度,只有 brimonidine (68 µM、6.8 µM) 會明顯降低細胞內鈣離子的濃度,而 Benzalkonium chloride 防腐劑對細胞內鈣離子的流動性沒有影響。綜合以上的結果顯示所有的抗青光眼藥可能會改變角膜內皮細胞內鈣離子濃度而影響細胞的生理功能。 |
英文摘要 | The aim of this study was to estimate the effects of various antiglaucoma drugs including betaxolol, timolol, levobunolol, brimonidine, carteolol, dipivefrin, dorzolamide, brinzolamide, latanoprost, unoprostone, and pilocarpine on intracellular free Ca2+ ([Ca2+]i) mobility in cultured bovine corneal endothelial cells. Various antiglaucoma drugs were diluted from original concentrations to 1/100, 1/1,000, and 1/10,000. The [Ca2+]i mobility was studied by spectrofluorophotometry after loading with the ester of fura-2 (fura-2/AM). It was found that timolol (58 µM and 5.8 µM), levobunolol (171 µM, 17.1 µM, and 1.71 µM), betaxolol (162 µM, 16.2 µM, and 1.62 µM), carteolol (680 µM and 68 µM), dipivefrin (28 µM and 2.8 µM), dorzolamide (616 µM and 61.6 µM), brinzolamide (260 µM), latanoprost (1.1 µM), unoprostone (28.2 µM, 2.82 µM, and 0.282 µM), and pilocarpine (408 µM and 40.8 µM) induced a significant increase in [Ca2+]i. Nevertheless, only brimonidine (68 µM and 6.8 µM) decreased [Ca2+]i concentration significantly. Benzalkonium chloride preservative did not affect [Ca2+]i after addition of 0.001, 0.0001 and 0.00001 mg/mL to cells. These results indicate that all antiglaucoma drugs may affect the physiologic function of corneal endothelial cells through change of [Ca2+]i mobility. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。