查詢結果分析
相關文獻
- Evaluation of Quantitative Analysis of Flavonoid Aglycones in Ginkgo biloba Extract and Its Products
- HPLC分析法在中草藥質量鑑定及其方法建立之進行模式
- 川芎 於大白鼠腦部各區之濃度分佈研究
- 茶葉及咖啡中咖啡因之含量分析
- 中藥方劑之品質管制(4)
- 市售柚類果實、果汁、及柚糖中柚皮苷之含量分析
- HPLC Assays of Naringin and Hesperidin in Chinese Herbs and Serum
- 中藥處方傳統煎劑之指標成分定量及其移行率之探討
- 川芎與鬱金所含川芎嗪之高效液相層析定量分析
- 利用高效液相層析法分析定量Quinolone類抗菌劑
頁籤選單縮合
題名 | Evaluation of Quantitative Analysis of Flavonoid Aglycones in Ginkgo biloba Extract and Its Products=銀杏浸膏及其製劑中三種類黃酮苷元定量方法之評估 |
---|---|
作者 | 秦玲; 林宜蓉; 黃成禹; 溫國慶; Chin, Lin; Lin, Yi-rong; Huang, Cheng-yu; Wen, Kuo-ching; |
期刊 | 藥物食品分析 |
出版日期 | 20001200 |
卷期 | 8:4 2000.12[民89.12] |
頁次 | 頁289-296 |
分類號 | 418.5 |
語文 | eng |
關鍵詞 | 銀杏葉浸膏; 高效液相層析法; Ginkgo biloba extract; HPLC; Quercetin; Kaempferol; Isorhamnetin; |
中文摘要 | 本研究針對有關銀杏葉浸膏原料及製劑內qucercetin, kaempferol 及isorhamnetin 之HPLC定量方法進行評估,結果顯示,分析條件使用Cosmosil 5C18-AR層析管,以MeOH : 0.3%H3PO4(1:1)為移動相,分離效果良好。各成分檢量線之相關係數分別為quercetin r = 0.9999 , kaempferol r = 0.9999 , isorhamnetin r =0.9989 ,而同日內檢測之相對標準偏差分別為0.29% , 0.28% ,及1.90% ,異日間檢測之相對標準偏差分別為0.95%,0.85% 及1.67% ,其添加回收率則分別為87.4% ,94.0% 及72.4% 。另外檢體前處理之方法,使用20%HCl溶液經15分鐘加熱迴流,與使用5.5%HCl溶液經30分鐘加熱迴流,可得到相近之水解效果。 |
英文摘要 | A simple and rapid HPLC method was developed to evaluate and validate the assay of quercetin, kaempferol and isorhamnetin in Ginkgo biloba extract and its products. The column used is Cosmosil 5C18-AR, and mobile phase is MeOH:0.3%H3PO4 (1:1). The correlation coefficients of linear calibration for quercetin, kaempferol and isorhamnetin are 0.9999, 0.9999, and 0.9989, respectively. The relative standard deviations of intra-day assays for quercetin, kaempferol, and isorhamnetin are 0.29%, 0.28%, and 1.90%, respectively; interday assays are 0.95%, 0.85%, and 1.67%, respectively. The recoveries of quercetin, kaempferol and, isorhamnetin are 87.4%, 94.0%, and 72.4%, respectively. The optimal condition for acid hydrolysis of flavonoid glycosides in Ginkgo biloba extract could be achieved by refluxing with 20.0% HCl at 85°C for 15 min or with 5.5% HCl at the same temperature for 30 min. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。