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頁籤選單縮合
題 名 | Qualitative and Quantitative Evaluation of Rice Component in Cereal Products by the DNA-based Technology=利用DNA分析進行穀物製品中米成分之定性與定量 |
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作 者 | 葉伶宜; 陳曉華; 陳倩琪; 仇志強; 傅偉光; | 書刊名 | 藥物食品分析 |
卷 期 | 11:1 2003.03[民92.03] |
頁 次 | 頁16-21+73 |
分類號 | 341.91 |
關鍵詞 | 米; PCR技術; 雜交反應; Rice; Polymerase chain reaction; PCR; Hybridization; |
語 文 | 英文(English) |
中文摘要 | 本研究利用DNA分析技術來對米及其製品作定性分析,並進一步評估利用此法推算米製品中米含量百分比之可行性。於定性方面使用PCR技術成功找到合適的引子 (primer) ,將未製品中斷裂且少量之米DNA定性出來。定量分析評估部份,找到一段對未具專一性之探針,嘗試利用此一探針做DNA雜交反應 (hybridization) 來估算樣品中來DNA含量,進而推算樣品中米含量百分比之可行性。以台糯70號米穀粉當作標準品做添加試驗,結果顯示本研究所使用之分析系統,其曲線迴歸之線性相關係數(R2值)達0.98,且自行調配已知比例之混合米穀粉樣品,經二重被分析結果之變異係數小於3%,表示利用分析樣品中糯米DNA的量來估算樣品中精米含量百分比的方法其誤差值在可接受範圍內。此分析方法目前受限於感度與標準品取得問題,僅可使用在生粉樣品上。由於不同製程所造成DNA斷裂的程度不同,所以若要使用此法於實際樣品之DNA定量分析,則需視不同米種與不同製程來選擇合適之標準品做迴歸內插,以降低誤差。 |
英文摘要 | A method based upon the polymerase chain reaction (PCR) has been developed for distinguishing rice species from other cereals. Two specific primer sets designed from 5S ribosomal RNA genes and promoter of rice glutelin genes were able to identify even small amount of DNA fragments in rice. DNA isolated from grain, flour and such products gave similar results. Furthermore, evaluation of quantitative analysis of rice in the samples was accomplished by hybridization with probe designed from promoter of rice glutelin genes. The analysis of a lab-made sample (a mixture of TCW70 rice flour and cassava starch) with nonradioactive labeling probe was performed. The R2 of calibration curve was 0.98 and the calculated amount of rice flour in the sample was very close to the actual value with CV < 3% (n = 2). However, this method is only proper for rice flour, because it is difficult, but necessary, to select a suitable standard that can exhibit rice species and broken situation of DNA about the samples. |
本系統中英文摘要資訊取自各篇刊載內容。