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題 名 | A Tissue Culture Protocol for Propagation of a Rare Plant, Lilium Speciosum Thunb. var. Gloriosoides Baker=組織培養法繁殖臺灣稀有植物--艷紅鹿子百合 |
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作 者 | 張正; 陳昌岑; 蔡瑜卿; 張唯勤; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 41:2 2000.04[民89.04] |
頁 次 | 頁139-142 |
分類號 | 435.456 |
關鍵詞 | 稀有植物; 麟莖; 癒傷組織; 艷紅鹿子百合; 麟片; Bulblet; Callus; Lilium speciosum thunb var gloriosoides baker; Rare plant; Scale; |
語 文 | 英文(English) |
中文摘要 | 艷紅鹿子百合幼花苞培植體在含 3 mg/l 2,4-D 及 0.25 mg/l BA 的 MS 基本培養基中三個月可以產生可繼代培養、具分化全能性的癒傷組織。將癒傷組織移植到含 0.1 mg/l NAA, 1 g/l 活性碳及 170 mg/l NaH2PO4 可以形成小鱗莖,繼而發育成為小苗。豔紅鹿子百合可藉由鱗片 -小鱗莖方法在試管內以 8 倍/三個月的速率大量增殖,經九個月的培養,己產生六千株試管內小苗。豔紅鹿子百合組織培養幼苗移植到溫室培養具 98% 存活率,栽培至第二年可抽莖及開花。 |
英文摘要 | Floret explant of a local accession of Lilium speciosum Thunb. var. gloriosoides Baker produced subculturable totipotent calli on a Murashige and Skoog basal medium with a supplement of 3 mg/l 2,4-dichlorophenoxyacetic acid and 0.25 mg/l benzyladenine. The calli were able to form bulblets, which subsequently developed into plantlets on the MS basal medium supplemented with 0.1 mg/l naphthalene acetic acid, 1 g/l active charcoal and 170 mg/l NaH2PO4. The rare lily was proliferated in vitro by a scale-bulblet cycling propagation method with a multiplication rate of eight times at three-month intervals. Finally, 6000 plantlets have been produced within nine months. We established 200 plants in the greenhouse under misty conditions for a four-week period with a 98% survival rate. These plants grew well and elongated with normal flowers in the second year. |
本系統中英文摘要資訊取自各篇刊載內容。