查詢結果分析
來源資料
相關文獻
- Demonstration of the Mycobacterium tuberculosis Complex Strain in Urine from a Patient with Suspected Urinary Tract Tuberculosis
- 利用PCR選殖落花生rDNA之IGS區域
- 氣功外氣對人類早期單核細胞株和流行性感冒病毒的效應
- Universal Primers for Amplification and Sequencing a Noncoding Spacer between the atpB and rbcL Genes of Chloroplast DNA
- 核酸偵測方法於草蝦白點病毒診斷上之應用
- 利用多型性增幅聚合酵素連鎖反應建立弧菌與其質體之分子分型
- 荔枝露疫病菌檢測用分子標記之建立
- Comparison of the Sensitivity and Specificity of an Automatic Ligase Chain Reaction Assay System with a One-Step Polymerase Chain Reaction Assay in the Diagnosis of Mycobacterium Tuberculosis Complex
- Determination of the Sensitivity and Specificity of PCR Assays Using Different Target DNAs for the Detection of Mycobacterium Tuberculosis
- Amplification of virD1 and Opine Synthase genes from Tumorigenic Rose and Aster Strains of Agrobacterium in Taiwan by Polymerase Chain Reaction
頁籤選單縮合
題 名 | Demonstration of the Mycobacterium tuberculosis Complex Strain in Urine from a Patient with Suspected Urinary Tract Tuberculosis=疑似尿路結核病患尿液檢體中含肺結核桿菌複合群的證明 |
---|---|
作 者 | 魏誠佑; 王文柄; 黃娟娟; 李展平; | 書刊名 | 慈濟醫學 |
卷 期 | 12:1 2000.03[民89.03] |
頁 次 | 頁17-24 |
分類號 | 415.2773 |
關鍵詞 | 肺結核桿菌; 聚合酵素連鎖反應; 尿路結核; Mycobacterium tuberculosis; Polymerase chain reaction; Urinary tract tuberculosis; |
語 文 | 英文(English) |
中文摘要 | 目的:尿路結核是由肺結核桿菌或肺結核桿菌複合群所引起的一種肺 外感染。因為肺結核桿菌生長緩慢、尿液檢體一般含極少菌數、傳統抹片染色與 細菌培養法的低敏感性,因此極需用分子診斷方法來偵檢與鑑定尿液檢體中的肺 結核桿菌複合群。病人及方法:尿液檢體在不同時間收集。核酸由尿液檢體純化 後,執行巢式聚合酵素連鎖反應。聚合酵素連鎖反應的產物純化後,執行核酸定 序反應以確認反應陽性的正確性。結果:第一次空尿與晚上收集的尿液檢體皆為 聚合酵素連鎖反應陽性;然而第一次空尿檢體的陽性訊號較晚上收集的尿液檢體 為強。結論:核酸定序反應證明反應的產物與已知的IS6110核酸序列相同,因 此確認其為肺結核桿菌複合群的感染。此分子診斷提供泌尿科醫師一個除了病患 的臨床症狀與尿液細菌培養結果以外的重要輔助方法,來診斷尿路肺結核桿菌複 合群的感染。 |
英文摘要 | Objective: Urinary tract tuberculosis is an extrapulmonary infection by Mycobacterium tuberculosis (M. tuberculosis) or the other M. tuberculosis complex strains. Due to the slow-growing characteristic of mycobacteria, very low numbers of mycobacteria present in urine samples, and low detection sensitivity by culture or the smear acid-fast stain method conventionally used for the urine sample, there is an acute need for ways to detect and confirm the M. tuberculosis complex in urine. Patients and Methods: Here we demonstrate the presence of M. tuberculosis complex in urine samples from a patient with suspected urinary tract tuberculosis, a representative case of extrapulmonary tuberculosis, by a nested polymerase chain reaction (PCR) assay and direct DNA sequencing. DNA was purified from urine samples collected at different times. The PCR product generated was purified, and then its sequence was determined. Results: Both the first-voided urine and urine sample collected at night from the patient with suspected urinary tract tuberculosis were PCR positive for M. tuberculosis complex strains; the first-voided urine sample showed a stronger PCR signal than the urine sample collected at night. Conclusions: The DNA sequence of the PCR product was demonstrated to be consistent with the known IS6110 insertion element found specifically in M. tuberculosis complex strains. This molecular approach provides urologists with an important adjunct, in addition to the patient's clinical presentation and urine culture data, for the diagnosis of urinary tract tuberculosis. |
本系統中英文摘要資訊取自各篇刊載內容。