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題 名 | Control of the Eel (Anguilla Japonica) Pathogens, Aeromonas Hydrophila and Edwardsiella Tarda, by Bacteriophages=噬菌體控制鰻魚(Anguilla Japonica)病原菌,親水性產氣單胞菌和愛德華氏菌之研究 |
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作 者 | 徐基新; 羅仲邑; 劉仲康; 林全信; | 書刊名 | 臺灣水產學會刊 |
卷 期 | 27:1 2000.03[民89.03] |
頁 次 | 頁21-31 |
分類號 | 439.5 |
關鍵詞 | 日本白鰻; 親水性產氣單胞菌; 愛德華氏菌; 噬菌體; Anguilla japonica; Aeromonas hydrophila; Edwardsiella tarda; Bacteriophages; |
語 文 | 英文(English) |
中文摘要 | 親水性產氣單胞菌(Aeromanas hydrophila)及愛德華氏菌(Edwardsiella tarda)為鰻魚最主要之病原菌。目前在養殖界控制病原菌的方式仍以施用抗生素等藥劑為主,然而在細菌抗藥性不斷增強下,發展另類拮抗物質有其必要性。在自然界中,噬菌體是造成細菌死亡的重要因子,本研究的目的在於了解噬菌體於自然水相溶裂宿主菌的能力,提供實際應用參考,以A. hydrophila ATCC 7966、E. tarda CCRC 10670及來自病鰻所分離之E. tarda為宿主,自南臺灣水域中分離出五株強溶裂型噬菌體,分別定名為A1、A2、E1、E2及T1。經初步鑑定,A2、E1、E2遺傳物質皆為雙股DNA且可以被限制?EcoR1切割。在實驗室中觀察觀察顯示,25℃下,在感染倍數(multiplicity of infection, moi)約等於11.5以上時,可在2小時內使菌數降低3次方。在養殖池水中,額外添加A. hydrophila至每毫升6 × 105菌數,在噬菌體A1的感染倍數為0.23時,在8小時內即將菌數降低250倍,同時產生高量的噬菌體,殘存的寄主大部分(85%)還是對病毒敏感。其餘在15%則具有抵抗性,其原因可能係為溶素原(lysogen)。而E. tarda 並不能在水體長存。在沒有添加噬菌體的情況下,即使額外添加菌數至每毫升4次方,終究會在48小時內菌數降低為原菌數之千分之一。 |
英文摘要 | Aeromonas hydrophila and Edwardsiella tarda are the two major pathogens of the eel, Anguilla japonica. The prevalent method to control the diseases is antibiotics. Long term and large scale application of the drugs results in resistance which makes disease control difficult. In the nature, bacteriophages are an important factor in controlling bacterial population. The purpose of this research is to study the capability of the phages to control the pathogens in pond water. Several bacteriophages of A. hydrophila and E. tarda were islated from the water samples of southern Taiwan. In pure culture, the phages could reduce the host 3 orders of magnitude in 2 hr when the multiplicity of infection (moi) was above 11.5 at 25℃. In the pond water with added A. hydrophila to 6 × 105/ml, the number dropped 250 folds at phage moi of 0.12 in 8 hr with accompanying phage multiplication to the level of 105 PFU/ml in the water. Most (85%) of the surviving hosts were still vulnerable to the phage. The resistant strains (15%) appeared to be lysogens since the culture broth of the strains could form phage plaques on A. hydrophila. In the case of E. tarda, the bacteria subsided rapidly even in the absence of phage in 45 hr in the pond water. |
本系統中英文摘要資訊取自各篇刊載內容。