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題名 | Design and Synthesis of an Activity Probe for Protein Tyrosine Phosphatases |
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作者姓名(中文) | 羅禮強; 王欣一; 王子健; | 書刊名 | Journal of the Chinese Chemical Society |
卷期 | 46:5 1999.10[民88.10] |
頁次 | 頁715-718 |
分類號 | 346.78 |
關鍵詞 | 香豆素; 螢光; 醌酪胺酸; Activity probe; Biocatalyst; Coumarin; Fluorescent; Mechanism-based selection; Protein tyrosine phosphatase; Quinone methide; |
語文 | 英文(English) |
英文摘要 | Protein tyrosine phosphatases (PTPases) are an important class of enzymes involved in the regulation of many cellular events. Here we describe the design and synthesis of an activity probe 2 targeting these PTPases. This mechanism-based activity probe adopts a cassette-like design; a phosphate group serves as the recognition head and a fluorescent diethylaminocoumarin derivative acts as the reporter group. Compound 3 was phosphorylated with diallyl phosphorochloridate and then fluorinated with DAST to give versatile intermediate 5. The Boc protective group of compound 5 was removed by TFA to make available the amino group where a diethylaminocoumarin chromophore was later attached. Final deprotection of the allyl group from the phosphate head gives our complete activity probe 2. It will be used in the labeling study of PTPases from various sources. |
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