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題名 | 不同條件下Rhodococcus equi No.23生產膽固醇氧化酶培養液中膽固醇顆粒大小及質量之分布=The Distribution of Cholesterol Particle Size and Mass in the Cholesterol Oxidase Producing Medium of Rhodococcus equi No.23 under Different Conditions |
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作者 | 李明聰; 周正俊; 陳文章; | 書刊名 | 中國農業化學會誌 |
卷期 | 37:4 1999.12[民88.12] |
頁次 | 頁523-532 |
分類號 | 346.78 |
關鍵詞 | 膽固醇氧化酶; 膽固醇顆粒粒徑分佈; 培養條件; Cholesterol oxidase; Size and distribution of cholesterol particles; Rhodococcus equi No.23; Culture conditions; |
語文 | 中文(Chinese) |
中文摘要 | 本研究乃在探討在不同條件下膽固醇氧化□產生菌,Rhodococcus equi No. 23培養液中,膽固醇顆粒大小與分佈之情形。以三角瓶為培養容器時,在震盪培養過程中膽固醇懸浮顆粒粒徑會隨著時間而逐漸變小,大約在培養6小時以後,顆粒粒徑分布會趨於穩定。以發酵槽為培養容器時,膽固醇顆粒粒徑也會隨著培養時間之延長而逐漸變小,並於攪拌培養約4小時後會趨於穩定。但以三角瓶培養時顆粒之平均粒徑比在發酵槽者高。膽固醇顆垃分布之分析顯示在所試之發酵槽培養條件下,培養液中實際可分散之膽固醇量約為0.8 g/L。以此為培養液中之膽固醇含量進行R. equi No.23之培養,可以提高膽固醇之利用率,並且可顯著地縮短發酵所需之時間。 |
英文摘要 | The distribution of cholesterol particle size and mass in the culture medium of the cholesterol oxidase producing Rhodococcus equi No. 23 under different culture conditions was investigated in this study. In a flask shaken culture, the cholesterol particle size decreased with the cultivation time. However, the distribution of cholesterol particle size became stable after 6 hr of cultivation. In a fermentor, the cholesterol particle size also reduced with time during the first four hours of cultivation. The average particle size obtained in the shaken flask was larger than that in the fermentor. Analysis of the cholesterol particle size in the fermentor revealed that maximally 0.8 g/L of cholesterol could be completely dispersed in the culture medium. Cholesterol could be utilized more effectively if the cultivation medium of R. equi No. 23 contained this amount of cholesterol, |
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