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題名 | 以ITSⅡ rDNA序列探討麴菌屬之各亞屬及其有性世代型菌種的類緣關係=Phylogenetic Relationships among the Sections of Form-genus Aspergillus and Their Teleomorphs Inferred from ITSⅡ rDNA Sequences |
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作者姓名(中文) | 汪碧涵; 王仕文; 王嬿婷; | 書刊名 | 中國農業化學會誌 |
卷期 | 37:4 1999.12[民88.12] |
頁次 | 頁470-480 |
分類號 | 379.13 |
關鍵詞 | 麴菌; 核醣體DNA; 間隔轉錄區; DNA序列; 無性世代型; 完整型; 親緣性; Aspergillus; Ribosomal DNA; Internal transcribed spacer; ITS; DNA sequence; Anamorph; Teleomorph; Phylogeny; |
語文 | 中文(Chinese) |
中文摘要 | 超過180種麴菌中,約有70種具有有性世代型,分別屬於子囊菌的八個屬中。本研究選用麴菌六個亞屬的代表菌種,及其對應之完整型子囊菌共十八株,以引子ITS1及ITS4增量核醣體核酸中的ITS I-5.8S-ITS II區,經純化後再以標幟螢光的引子ITS3與ITS4,用核酸定序儀分別做ITS II區域正向與反向的定序,再將所定出的序列輸入電腦,以eurospora crassa 與 Talaromyces flavus做為外群,用軟體PAUP 3.1.1分析,並加入一些已發表的相關菌種序列,繪製系統樹狀圖。類緣分析結果顯示,麴菌及其完整型菌株,被分在同一分支,以bootstrap值77與T.flavus分開,支持Aspergillus為同源真菌的假說。ITS II的核酸序列可用以區分五個麴菌亞屬,分別代表Aspergillus glaucus. Aspergillus nidulans, Aspergillus ochraceus. Aspergillus flavipes, Aspergillus ornatur 亞屬的五個菌株:A. amstelodami, A. nidulans, A. alliaceus. A. flavipes及A. paradoxus等均與其對應之完整型菌株分別屬於同一分支,亞屬內,無性世代型與完整型的核酸序列都有很高的相似性。Aspergillus flavipes的ITS II區域有種內變異出現,但是無性世代型和完整型的相似度高於麴菌亞屬之間的相似度。Aspergillus fumigatus亞屬是唯一的例外,A. aureolus與完整型菌株N. aureola的類緣關係較其它亞屬的無性世代型與完整型疏遠,Aspergillus fumigatus的有性世代是否屬於Neosartoya屬,需要更多的證據。 |
英文摘要 | The genus Aspergillus contains more than 180 species, with about 70 named teleomorphs. These teleomorphs are placed in at least eight genera of Ascomy-cotina. In this study, 18 isolates of Aspergillus species assigned to 6 teleomorphs of Aspergillus and their anamorphs, including reference isolates, were studied using sequence analysis of the rDNA internal transcribed spacer II(ITS II) region. The related species Neurospora crassa and Talaromyces flavus served as outgroups for the analysis. The determined sequences were aligned and analyzed using PAUP 3.1.1. A phyolgenetic tree was reconstructed using the Neighbour-joining method with the inclusion of sequence of some published related species. The branch sepa-rating T. flavus and Aspergillus spp. was supported by 77% bootstrap support. Aspergillus isolates and their holomorphs were grouped together. These results supported the hypothesis that Aspergillus is monophyletic. When the similarity between Aspergillus species and their teleomorphs was compared and the phyolgenetic relationships between those genera was determined, A. amstelodami, A. nidulans. A. alliaceus, A. flavipes, and A. paradoxus, which represented sections of A. glaucus, A. nidulans, A. ochraceus, A. flavipes and A. ornatus, were considered to be phyolgenetically related to their holomorphs both separately and as cluster in five sections. Using ITS II sequence analysis, anamorphs among their closed holomorphs could be placed. Among group A. flavipes, three A. flavipes isolates and three holomorph isolates were clustered into two subsets together. Phyolgenetic comparison showed the ITS II region of A. flavipes has intraspecific variation, however, relationship between anamorph and teleomorph was closer than the relationship between different Aspergillus sections. The only exception was Aspergillus fumigatus section, a high degree of variation was evident between isolates of A. aureolus and teleomorph isolate N. aureola. |
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