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題 名 | 引起筒蒿白化嵌紋病之蕪菁嵌紋病毒分離株之寄主反應、細胞學特性及血清學性狀=Host Reactions, Cytological Characteristics and Serological Properties of a Turnip Mosaic Virus Isolate Causing Albinic Mosaic Disease of Garland Chrysanthemum |
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作 者 | 陳翠妙; 陳煜焜; 陳脉紀; 葉錫東; | 書刊名 | 植物病理學會刊 |
卷 期 | 8:2 1999.06[民88.06] |
頁 次 | 頁73-82 |
分類號 | 435.2 |
關鍵詞 | 茼蒿白化嵌紋病; 蕪菁嵌紋病毒; 血清學; 細胞學; Garland chrysanthemum albinic mosaic virus; Turnip mosaic virus; Serology; Cytopathology; |
語 文 | 中文(Chinese) |
中文摘要 | 茼蒿白化嵌紋病毒(garland chrysanthemum albinic mosaic virus, GCV-Am)造 成茼蒿矮化,葉片嵌紋,白化或裂葉等病徵,為一種長絲狀病毒,大小約 775 × 13nm,經 鑑定屬於蕪菁嵌紋病毒 (turnip mosaic virus, TuMV) 之一分離株。 為釐清 GCV-Am 與其 他 TuMV 系統之關係,本研究特將其與 TuMV 之 C1,C2,C3,C4 及 C5 病毒系統的寄主反 應、 細胞學特性及血清性狀做一詳細比對。 以 GCV-Am 接種四種白菜鑑別品種 (B campestris ssp. pekinensis cutivars):Tropical Delight, Crusader, PI418957 及 PI419105,發現均可感染產生病徵,與本省 TuMV-C5 系統特性相似。 由細胞學特性之比較 中,發現 GCV-Am 與其他 TuMV 系統均可在細胞質內產生圓柱狀內含體,形態包括風車狀、 薄層狀、卷軸狀及束狀內含體。 為釐清 GCV-Am 與各系統間之相關性, 分別利用本省五種 TuMV 病毒系統 (C1、C2、 C3、C4 及 C5) 及 GCV-Am 病毒與 GCV-Am 抗血清、臺灣蘿蔔分 離株 TuMV-R 抗血清及德國甘藍分離株 TuMV-B 抗血清進行免疫雙向擴散反應,結果均可產 生親和性之沈澱帶:而以間接酵素連結免疫吸附法測試,此六種抗原與 GCV-Am 之抗血清及 德國 TuMV-B 抗血清之反應強度均相近。西方轉漬法測試亦均有相同之反應條帶且六者之鞘 蛋白分子量均為 35kDa。本研究之結果顯示 GCV-Am 與五種 TuMV 系統間無法以血清學或細 胞學等方法明顯區分,但依寄主特性顯示 GCV-Am 屬於 TuMV-C5 系統。 |
英文摘要 | A disease of garland chrysanthemum (Chrysanthemum coronarium var. spatiosum Bailey) showing symptoms of albinism, mosaic, cleavage of leaves and stunted growth was found in the central part of Taiwan. A filamentous potyvirus (named GCV-Am) about 775 × 13nm in size was isolated from the infected plants and proved to be the causal agent of the disease. In this investigation, it was further compared with the C1,C2,C3,C4, and C5 strains of turnip mosaic virus (TuMV) by host reactions, cytological characteristics and serological properties. GCV-Am induced symptoms on Brassica campestris subsp. pekinensis cultivars Tropical delight, Crusader, PI418957 and PI419105, similar to those induced by the TuMV C5 strain originated from Taiwan. All isolates of the TuMV strains used in current studies induced cylindrical inclusions in host cytoplasm, in the shapes of pinwheels, laminated aggregates, scrolls and bundles. In immunodiffusion tests, coalescent precipitin lines without spurs were observed when antisera to GCV-Am, a Taiwan radish TuMV-R isolate, and a cabbage TuMV-B isolate from Germany were used to react with crude antigens from mustard infected with GCV-Am, TuMV-R, or the C5 strain. Also, similar results were noticed when the antiserum to GCV-Am was used to react with crude antigens of homologous GCV-Am and heterologous C1,C2,C3,C4 and C5 strains. In indirect ELISA and western blotting tests, the antisera to GCV-Amor TuMV-B also reacted with teh GCV-Am isolate and the other five strains, indistinguishably. the molecular size of the coat protein of GCV-Am was found to be 35kDa similar to those of other five strains. The results of this study indicated that GCV-Am belongs to the C5 strain of TuMV based on host reactions, but indistinguishable from othe TuMV strains from Taiwan in cytopathological characteristics and serological properties. |
本系統中英文摘要資訊取自各篇刊載內容。