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頁籤選單縮合
題名 | Serological Characterization of Papaya Ringspot Virus Isolates in Taiwan=木瓜輪點病毒系統之血清學特異性 |
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作 者 | 關政平; 陳光慧; 蘇鴻基; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 40:3 1999.07[民88.07] |
頁次 | 頁231-236 |
分類號 | 435.354 |
關鍵詞 | 植物病毒; 木瓜輪點病; 血清學; Papaya ringspot disease; Plant virus; Serology; |
語文 | 英文(English) |
中文摘要 | 木瓜輪點病毒由國內分離出 27 個不同型系統分離株 , 發現木瓜輪點病毒病徵型系統之變異 , 包括壞疳成紋型、重嵌紋型、輕嵌紋型外,尚有畸型成紋型等系統, 這些木瓜病毒與另一非木瓜系統之西瓜嵌紋病毒第一型,同時在矮南瓜病徵表現做比較。利用製備之多元抗體,無法區分所有木瓜輪點病毒分離株和西瓜嵌紋病毒, 而以製備之單元抗體系列則可區分。一些病徵型與單元抗體血清型反應型不儘吻合, 由各單元抗體與 27 個不同型系統分離株利用酵連抗體免疫標誌法之反應型可歸納ll株單元抗體為5群, 推 測此病毒至少其 5 種判讀抗原基 , 使用單元抗體有利於鑑定瓜類中病毒系統。 |
英文摘要 | Twenty-seven isolates of papaya ringspot virus (PRSV) were obtained from Taiwan. Differences in symptoms were observed. In addition to such isolates as necrotic severe mottle (SMN), severe mottle (SM) and mild mottle (M), another mottle type with deformation (DF) was isolated. All the isolates of PRSV and PRSV-W (PRSV type W) examined were serologically indistinguishable as determined by polyclonal antibodies-rabbit antisera to PRSV, but were consistently distinguishable with monoclonal antibodies (MAbs). Those isolates were raised against the PRSV. The virus isolates that reacted with the MAbs to PRSV were selected and applied to compare the antigenic specificity of the PRSV strain. Moreover, the immunoreactivities of the MAbs were compared with polyclonal rabbit serum to detect PRSV in foliar tissue by enzyme-linked immunosorbent assay (ELISA). Eleven selected MAbs were differentiated into five sero-groups according to sero-reaction patterns of ELISA index with 27 isolates of PRSV and PRSV-W. It was assumed that at least five antigenic epitopes were identified. Distinct results were obtained in application of MAb to ELISA for detecting PRSV strains in cucurbitaceous plants. |
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