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- Effect of Sorbitol Induced Osmotic Stress on the Changes of Carbohydrate and Free Amino Acid Pools in Sweet Potato Cell Suspension Cultures
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頁籤選單縮合
題 名 | Effect of Sorbitol Induced Osmotic Stress on the Changes of Carbohydrate and Free Amino Acid Pools in Sweet Potato Cell Suspension Cultures=山梨糖醇誘導的滲透逆境對甘藷懸浮培養細胞碳水化合物與自由態胺酸庫變化的影響 |
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作 者 | 王恆隆; 李平篤; 劉麗飛; 蘇仲卿; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 40:3 1999.07[民88.07] |
頁 次 | 頁219-225 |
分類號 | 463.36 |
關鍵詞 | 可親和性溶質; 自由態胺酸; 甘藷; 滲透逆境; 澱粉; 蔗糖; 懸浮細胞; Compatible solute; Free amino acid; Ipomoea batatas; Osmotic stress; Starch; Sucrose; Suspended cells; |
語 文 | 英文(English) |
中文摘要 | 本研究探討高濃度 (0.6M) 山梨糖醇引起的滲透逆境 , 對於甘諾 ( 台晨 57 號 ) 懸浮培養細胞的生長 以及碳水化合物和自由態胺酸在定性與定量方面的影響。比較細胞分別移入培養基不合 ( 正常處理 ) 與合 ( 逆境衝擊處理 ) 山梨糖醇 ' 以及細胞長期培養在高滲透逆境下 ( 逆境適應處理 ) 。逆境衝擊阻礙細胞生長 並且造成原生質分離。相關令逆境衝擊的細胞 , 適應逆境的細胞具有較短的生長遲滯期和正常型態 , 雖然 稍小於正常處理的細胞。在逆境衝擊下 , 細胞內胺酸庫增加 4 倍 , 而以丙胺酸、要去胺酸及其衍生物之增 加為顯著 , 顯示糖解代謝之改變對胺酸之合成發生影響。雖然鵬胺酸的含量亦增加 5 倍 , 由於僅佔總胺 酸的 1. 凱 , 無法說明具有顯著的生理意義。在碳水化合物方面 , 高含量的蕪糖存在於逆境衝擊與逆境適 應的細胞 ; 而大量澱粉累積僅發生於受逆境衝擊的細胞 , 未表現於正常處理與適應逆境的細胞 , 雖然後者 亦有稍高的澱粉含量。因此 , 合理推測蕪糖當作可親和 | 生溶質 , 而且蕪糖轉換成澱粉的機轉在調節高滲透 環境中扮演關鍵角色。由滲透逆境所累積澱粉的直鏈澱粉含量明顯低於來自新鮮甘藉塊根 , 表示遭受逆境 衝擊的細胞在合成澱粉的途徑具有些許改變。 |
英文摘要 | The effects of osmotic stress induced by 0.6 M sorbitol on the cell growth and on the quantitative and qualitative changes in carbohydrates and free amino acids in suspended cells of sweet potato (Ipomoea batatas) were analyzed. Cells transferred into medium without (normal treatment) or with (stress-shocked treatment) 0.6 M sorbitol added, and cells consecutively subcultured under high stress conditions (stress-adapted treatment) were compared. Stress-shocked cells showed cell growth retardation and the induction of plasmolysis. Stress-adapted cells had a shorter lag phase in growth than the stress-shocked, and showed a normal morphology, albeit the size appeared slightly smaller than normal cells. Under the stress-shocked condition, the size of the amino acid pool (μmole/g fresh weight) increased fourfold relative to the control and stress-adapted cells. The levels of alanine and glutamic acid and its derivatives were especially high, indicating that the changes in the intensity of glycolysis have influenced the amino acid pool. Although the proline level showed a fivefold increase when stress-shocked, proline made up only about 1.5% of total amino acids, and thus did not seem to play an osmotic regulatory function. Among the carbohydrates, sucrose content was high in both stress-shocked and stress-adapted cells. Starch accumulated heavily in stress-shocked cells, but not in normal or stress-adapted cells, although the latter maintained a higher background level of starch. It is tempting to speculate that sucrose serves as a compatible solute, and starch synthesis from sucrose plays a pivotal role in moderating the hyperosmotic condition. The accumulated starch contained less amylose than the ordinary tuberous root starch, indicating that the pathway of starch synthesis was somewhat altered in the stress-shocked cells. |
本系統中英文摘要資訊取自各篇刊載內容。