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題 名 | 中藥(材)中微量元素之檢驗方法及規格研訂4.--礦物中藥(材)中微量元素(銅、汞、鎘、鉛、砷、鈷、錳)檢驗方法及規格研訂之研究=Analysis and Specification of Trace Elements in Chinese Herbal Medicines.--Analysis and Specification of Trace Elements (Cu,Hg,Cd,Pb,As,Co,Mn)in the Minerals of Chinese Herbal Medicine |
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作 者 | 郭宗禮; | 書刊名 | 中醫藥年報 |
卷 期 | 17:1 1999.05[民88.05] |
頁 次 | 頁259-278 |
分類號 | 414.33 |
關鍵詞 | 礦物性中藥材; 微量元素; 砷及汞定量; 氫化原子吸光光譜法; Mineral and animal chinese herbs; Trace elements; Arsenic and mercury determination; Hydride generation atomic absorption spectrophotometry; |
語 文 | 中文(Chinese) |
中文摘要 | 由於中藥生產地之不同,因土壤成份、重金屬農藥之使用、環境污染之差異或 是人為加工等,皆會造成各地中藥材重金屬含量之差異。由於重金屬所造成急性、慢性中毒 之事件,時有所聞,也有一些研究報告。因此,中藥材某些微量元素,是否與藥效有關? 如 長期使用是否也具有慢、急性毒性? 特別是中藥材所含重金屬之量是否會危害人體健康,實 有深入研究,及廣泛檢驗之必要。目前台灣中藥材之品質管制,尚未建立完整的科學化之重 金屬質量標準。而大陸方面,有關中藥材之重金屬定量,不但項目有限, 其方法也不夠嚴謹 ,未能達到現代化之國際水準。近來,由於儀器分析之進展及微量分析方法之開發,許多元 素之定量可達到超徽量之水準。因此,本研究由開發最佳微波硝化之前處理方法、經不同之 氫化儀器、再聯接到傳統之原子吸光儀,克服了由礦物性中藥材定量砷、汞之難題。此定量 法之標準曲線之線性相當良好,當砷濃度在 0.2-2.0ppb,汞濃度為 2.0-20ppb 時,其線性 之相關係數了 r=0.999 以上,且其準確性及回收率亦相當理想, 其標準誤差小於 3% 以下 。利用此方法分析中藥材之砷汞含量,也獲得很好之結果。此方法應可推廣應用到其它中藥 材之神汞定量,並進一步探討這些含砷、汞之中藥對生物體之影響,以作為政府將來訂定中 藥材規格時, 所需建立檢驗方法之標準檢驗流程 (SOP,standard operation protocol) 之 參考。 |
英文摘要 | Chinese herbal medicine, the most important part of traditional medicine, has been used by people since antiquity for the prevention and treatment of illness, is less well known in the West. It has been known that many herbs can be helpful and others might be potentially harmful. In the last decade, the herbal preparation, resources, identification, physiochemical evaluation, active principles and pharmacological activities have been studied. However, little knowledge is available about the role of trace elements in Chinese herbals. On the other hand, some cases of intoxication with arsenic, mercury and lead resulted from the ingestion of Chinese herbals have been reported recently. Therefore, in order to clarify the role of trace elements and to monitor the safety of herbal remedies, the analysis of tracd elements is very important for the pharmacological and toxicological evaluation of Chinese herbals. However, it is still a challenging task due to the matrix interferences of herbs. In this study, we developed a simple, sensitive and accurate method to determine and mercury in mineral and animal Chinese herbs. The specimens were pretreated with microwave digestion using nitric acid, and subsequently determined with hydride generation-atomic absorption spectrophotometry (HG-AAS) in conjunction with a flow injection analysis system (FIAS). The linearity of standard curves of these elements was excellent with correlation coefficients, r > 0.999. The detection limits are 0.2 ng/mL. On the other hand, the precision and recovery tests of arsenic and mercury by the proposed method are acceptable with coefficient of variations less than 3%. Some specimens of mineral and animals Chinese herbs were quantificated by the proposed method. Arsenic and mercury concentrations of these herbs were found in the ranges of 75.3 to 31,053.4 ng/g. The proposed method could be applied to analyzed other species of Chinese herbs and to evaluate their pharmacological and toxicological effects. |
本系統中英文摘要資訊取自各篇刊載內容。