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題 名 | Rapid Detection of Herpes Simplex Virus by Polymerase Chain Reaction=利用聚合酶連鎖反應對單純疱疹病毒作快速偵測 |
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作 者 | 王乃玉; 廖國楨; 翁麗涓; 邱玉英; 黃富源; 楊定一; 汪春雪; | 書刊名 | 微免與感染雜誌 |
卷 期 | 32:2 1999.06[民88.06] |
頁 次 | 頁99-104 |
分類號 | 414.84 |
關鍵詞 | 聚合酶連鎖反應; 單純疱疹病毒; Herpes simplex virus; HSV; Polymerase chain reaction; PCR; Cerebrospinal fluid; CSF; Genital swab; Throat swab; |
語 文 | 英文(English) |
中文摘要 | 本報告使用三組不同的引子,以聚合�t連鎖反應法(PCR),對單純�秤l病毒(Herpes simplex virus, HSV)作快速偵測,此三組 PCR 引子分別來自 HSV 基因體上的去氧核醣核酸聚合酵素基因(DNA polymerase gene)、醣蛋白B基因(glycoprotein B gene)和醣蛋白D基因(glycoprotein D gene)。我們將此三組 PCR 引子應用在來自咽喉及生殖部位的檢體,診斷是否感染HSV,實驗結果顯示這三組引子具有相似的敏感性,可以對相同的檢體偵測到HSV DNA,若以傳統的細胞培養方式作為標準,PCR 方法的敏感性及特異性,均可達到 100%。當我們將此三組 PCR 引子應用在來自疑似感染單純�秤l病毒腦炎(HSE)的病人之腦脊髓液(CSF)時,此三組PCR引子均無法偵測到任何HSV DNA。最後, 我們使用來自HSV基因體上的glycoprotein D gene之兩對引子,對這些CSF檢體進行 nested-PCR反應,發現10個病人檢體中有5個受到HSV的感染。由本實驗得知,PCR可作為快速診斷檢體中之HSV DNA的方法,尤其是針對不容易以組織培養出病毒的CSF檢體。 |
英文摘要 | This study reports the successful use of three sets of primers, each from different genes on the herpes simplex virus (HSV) genome, 1) the DNA polymerase gene, 2) the glycoprotein B gene, and 3) the glycoprotein D gene, for detection of HSV DNA by polymerase chain reaction (PCR). All three sets of primers detected the same HSV DNA in the throat and genital specimens. Using the conventional viral culture as a standard, PCR provided a sensitivity of 100% and a specificity of 100% in this study. In addition, a nested-PCR protocol using two sets of primers in the glycoprotein D gene, one set internal to the other, was evaluated for the amplification of HSV DNA in cerebrospinal fluid (CSF) from patients suspected of having herpes simplex encephalitis (HSE). Five of the 10 CSF specimens tested were found positive. In conclusion, PCR detection is a valuable tool for rapid diagnosis of HSV infection, especially for CSF specimens. |
本系統中英文摘要資訊取自各篇刊載內容。