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題名 | Effect of Liposomes on Mineralization in Rat Osteoblast-Enriched Cultures=微脂粒在大白鼠造骨母細胞培養中對骨質礦物質化之影響 |
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作者 | 黃景勝; 吳樺芬; 洪堅銘; 張富雄; 洪基隆; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷期 | 15:4 1999.04[民88.04] |
頁次 | 頁187-194 |
分類號 | 415.121 |
關鍵詞 | 微脂粒; 造骨母細胞; 礦物質化; Liposomes; Mineralization; Osteoblast; |
語文 | 英文(English) |
中文摘要 | 微脂粒是由人工合成,具有雙層磷酸脂分子構成的球體。內部可包覆水溶液而與外界區隔。本研究係應用微脂粒可以產生鈣化,因此可被用來研究扮涳骨質鈣化核心的基質泡之特性以了解微脂粒對造骨母細胞的生長以及礦物質化的影響。實驗取自Sprague-Dawley品系大白鼠21天大的胚胎之顱骨細胞,培養在35mm培養皿中,細胞培養液內加入含有100μM/L的微脂粒,微脂粒的組成為egg phosphatidylcholine,cholesterol及bovine brain phosphatidylserine,以後每兩天換一次培養液,實驗組分成增生組及鈣化組,增生組在16天內計算細胞數目與ALP活性。鈣化組在12至24中用von Kossa氏法染色之以供分析。用Two-way ANOVA分析結果知微脂粒對造骨母細胞的培養中,不影響細胞的增生,卻可以增加骨質鈣化,進而加速類骨組織的生成。 |
英文摘要 | Liposomes, artificial membranous lipid vesicles, have been used as model structures for biological calcification processes. However, there is no definite conclusion that liposomes can be like matrix vesicles for inducing bone calcification and bonelike tissue formation on primary cultured cells. To determine whether liposomes can promote bone cell growth and mineralization by inducing crystal nucleation, liposomes composed of egg phosphatidylcholine, cholesterol, and bovine brain phosphatidylserine were added to 21-day-old Sprague-Dawley fetal rat calvarial cell cultures from day 1. The aims were to observe proliferation and the phenotype of osteoblasts by measuring cell numbers and alkaline phosphatase (ALP) activity and, when added at confluence, to observe calcification. The data were analyzed with two-way ANOVA. During the 16-day culture period, cell numbers were not significantly affected by liposomes (100μ mol/L). However, ALP activity was significantly inhibited by liposomes (p<0.05) at day 16 and thereafter. Calcified particles were detected by von Kossa's method, and were larger and more abundant (p<0.05) in the liposomes groups than in the control from days 12-24. This response depended on liposomes dose. These findings suggest that liposomes promote calcification and accelerate the formation of bone-like tissue. Liposomes slightly reduce the expression of the osteoblast phenotype and do not affect cell growth in primary rat osteoblast-enriched cultures. |
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