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題 名 | 利用限制酶切割片段圖法分析臺灣地區的恙蟲病立克次菌株=Molecular Typing of Taiwanese Orientia tsutsugamushi Isolates by Restriction Fragment Profile |
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作 者 | 陳慧玲; 邱淑君; 陳豪勇; 王躬仁; | 書刊名 | 微免與感染雜誌 |
卷 期 | 32:1 1999.03[民88.03] |
頁 次 | 頁68-72 |
分類號 | 414.84 |
關鍵詞 | 恙蟲病立克次菌; 病原體型別分析; 限制酶切割法; Orientia tsutsugamushi; Typing of organism; Restriction enzyme digestiion; |
語 文 | 中文(Chinese) |
中文摘要 | 恙蟲病立克次菌所引起的恙蟲病是一種急性出疹熱病,在我國屬報告傳染病。本 研究係自急性期患者血液中利用細胞培養方式分離出71株病原體,經兩階段聚合�t 連鎖反應增殖具特異性的56KDa蛋白的部分去氧核醣核酸片段,再以Hha I及Sfa NI二種限制�t切割分型,並與於日本所分離出的Karp、Kato、Kawasaki、kuroki 、Gilliam及Shimokoshi等6株國際標準株比對後,發現有34株類似Kuroki 型,7株類似Karp型及2株類似Gilliam型,另有28株因為和此6株標準株均不相 同而暫時歸類命名為由Taiwan A到Taiwan F等6種新型,其中又以Taiwan A型 的23株最多。這些新型的出現,顯示國內可能存有特殊的本土變異株。 |
英文摘要 | Scrub typhus, which is caused by Orientia tsutsugamushi, is one of the reportable diseases in Taiwan, ROC. Positive cases seemed to be increasing in recent years and our laboratory had confirmed a total of 247 cases in 1997. In this study, 71 strains of O. tsutsugamushi were isolated from heparinized blood of the patients by cell culture, and their DNA was isolated. Two-step polymerase chain reaction was then used to amplify a specific sequence from the DNA, which was digested with restriction enzymes, Hha I and Sfa NI, for typing. With this technique, 34 strains gave rise to the same profile as that of the Kuroki type, 6 as the Karp type and 2 as the Gilliam type. The remaining 28 strains produced fragment profiles different from those of types Karp, Kato, Kawasaki, Kuroki, Gilliam and Shimokoshi and, therefore, were classified as 6 new types designated Taiwan A to Taiwan F, respectively. These strains with profiles different from those of the standard Japanese strains may represent the local mutant strains. |
本系統中英文摘要資訊取自各篇刊載內容。