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頁籤選單縮合
題名 | Use of Universal Polymerase Chain Reaction Assay and Endonuclease Digestion for Rapid Detection of Neisseria Meningitidis=以共同性聚合酶連鎖反應與限制酶切割法快速偵測腦膜炎雙球菌 |
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作者姓名(中文) | 林鴻瑋; 尹居浩; 樓震平; 王怡惠; 李詩益; 盧章智; | 書刊名 | 微免與感染雜誌 |
卷期 | 37:6 2004.12[民93.12] |
頁次 | 頁371-374 |
分類號 | 415.9313 |
關鍵詞 | 共同性聚合酶連鎖反應; 限制酶切割法; 腦膜炎; 雙球菌; DNA primers; DNA restriction enzymes; Meningitis; Neisseria meningitids; Polymerase chain reaction; |
語文 | 英文(English) |
英文摘要 | Neisseria meningitidis is a major cause of bacterial meningitis worldwide, especially in children. Early diagnosis and empiric antibiotic treatment have led to a reduction in morbidity and mortality. The value of the traditional gold standard diagnostic tests, blood culture and cerebrospinal fluid (CSF) culture, has been adversely affected by preadmission use of parenteral penicillin and fewer lumbar punctures. We report a case of N. meningitidis in a 19-year-old male who was admitted after suffering from progressive severe headache, and intermittent high fever for 2 days. Gram stain and culture of CSF, and culture of throat swab were negative. However, N. meningitidis was detected by polymerase chain reaction (PCR) with a universal primer set and endonuclease digestion. This report indicated that the PCR method may be an alternative method for the rapid diagnosis of meningococcal meningitis. |
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