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題 名 | Different Densities of Human Eosinophils Respond Differently to PAF and IL-5=不同比重的嗜伊紅性白血球對血小板活化因子及第五介白質的不同反應 |
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作 者 | 蔡肇基; 王亭方; | 書刊名 | 微免與感染雜誌 |
卷 期 | 32:1 1999.03[民88.03] |
頁 次 | 頁21-25 |
分類號 | 414.82 |
關鍵詞 | 嗜伊紅性白血球; 血小板; 活化因子; 第五介白質; Eosinophils; Platelet activating factor; Interleukin-5; |
語 文 | 英文(English) |
中文摘要 | 人體週邊血液中的嗜伊紅性白血球的功能有很大的差異,這些差異可能由於所含 之顆粒多寡及比重之高低而有所不同。嗜伊紅性白血球被活化可用染顆粒蛋白ECP 之單株抗體EG�砟峓t會分解螢光物質之水解酵素活性來加以評估,這兩種機能都可 以用細胞流速分析儀分析。本研究乃在探討不同比重之嗜伊性紅白血球對血小板活 化因子(PAF)及第五介白質(IL-5)之反應。結果顯示:低比重之嗜伊紅性白血球比 正常比重者較容易被EG�祝V上(88.1±8.4%及50.1±0.9%,p<0.05),當分析吞噬 反應後,產生水解汛之活性時,低比重之嗜伊紅性白血球帶有較高水解汛活性。這 兩種活性都可以被PAF及IL-5來促進,而低比重的嗜伊紅性白血球較正常比重者 更容易被PAF及IL-5來增強。結論為不同比重之嗜伊紅性白血球吞噬反應產生的 水解汛含量不同,這個活性可以被PAF及IL-5來增加。 |
英文摘要 | Human eosinophils are heterogeneous, consisting of both normal and light density cells which may differ in their functional properties. In this study, we compared different densities of eosinophils in response to PAF and IL-5. The eosinophil activation markers were identified either by staining with monoclonal antibody EG�� or, by respiratory burst activity with dichrofluorescin diacetate (DCFH-DA). Both functions were analysed by flow cytometric analyzer. Results showed that light density eosinophils stained with a higher percentage of EG �� (EG �� / Kimura stain)in comparison with normal density eosinophils (88.5 ± 19.1% vs. 43.9 ± 18.5% p <0.01). When both groups of cells were analysed with the respiratory burst activity, on EG �� + cells, the activity in light density EG �� +cells were much higher than that of normal density EG �� +cells. These activities in both groups of cells can be further enchanced by PAF and IL-5. Furthermore, the light density EG �� +cells were more eligible to PAF and IL-5 stimulation than were normal density EG �� +cells. In conclusion, normal density and light density eosinophils had different respiratory burst activities; both groups of EG �� +eosinophils responded differently to PAF and IL-5. |
本系統中英文摘要資訊取自各篇刊載內容。