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頁籤選單縮合
題名 | Isolation and Characterization of a Novel Peptide Gene in the Lobster Jasus Edwardsii=分離及描述一個龍蝦(Jasus edwardsii)新胜冴類基因 |
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作者姓名(外文) | Khoo,Jenny G. I.; Sin,Frank Y. T.; | 書刊名 | 動物研究學刊 |
卷期 | 38:1 1999.01[民88.01] |
頁次 | 頁95-109 |
分類號 | 387.13 |
關鍵詞 | 龍蝦; 後葉加壓素類似基因; 蛻皮抑制激素; 眼柄; 金屬硫蛋白類似蛋白質; Lobster; Vasopressin-like gene; Moult-inhibiting hormone; Eyestalk; Metallothionein-like protein; |
語文 | 英文(English) |
中文摘要 | 過去的研究顯示甲殼類中的後葉加壓素與脫皮抑制激素之間有著免疫系統及功 能上的相關性。利用由大鼠後葉加壓素基因序列所設計的引子,兩個片段可田龍蝦(Jasus edwardsii)基因組的脫氧核醣核酸被增殖到。這篇文章在描述對96Obp序列的分析。北方 氏點漬法結果顯示此96Obp序列表現在上皮、眼柄、心臟、肝胰臟及肌肉,但是表現主要 是在眼柄。利用96Obp序列為探針所進行的原位雜合反應可偵測到核醣核酸分而在眼柄之 神經分泌的區域。這些基因的表現在已蛻皮的眼柄比蛻皮中的眼柄要高。對96Obp聚合□ 連鎖反應產物序列分析的結果顯示有一表現序列/內子接合點,一蛋白質編碼區域具有一 連串重覆序列並可轉譯出一金屬硫蛋白類似蛋白質。同時,此96Obp序列亦與大鼠後葉加 壓素及一些甲殼類的MlH,CHH基因有人於百分之四十序列上相似性。我們稱呼此序列為新 性狀序列(NPS)。 |
英文摘要 | Jenny G.I. Khoo and Frank Y.T. Sin (1999) Isolation and characterization of a novel peptide gene in the lobster Jasus edwardsii. Zoological Studies 38(l): 95-109. Previous studies have demonstrated an immunological and functional relationship between vasopressins and the moult- inhibiting hormofie (MIH) of crustaceans. Using primers derived from the rat vasopressin gene, 2 fragments were amplified from the genomic DNA of the lobster Jasus edwardsii. This paper describes the characterization of the 960-bp sequence. Northern blot analysis showed that the 960 bp sequence was expressed in the epithelia, eyestalk, heart, hepatopancreas, and muscle, but expression was most prominent in the eyestalk. In situ hybridization using the 960-bp sequence as a probe detected RNAs in the neurosecretory regions of the eyestalk. The expression of these genes appeared to be higher in the intermoult than the ecdysial eyestalk Sequence analysis of the 960-bp PCR product revealed an intron/exon splice junction. a protein coding reqion with a stretch of repetitive sequences which is translated into a metallothionein-like protein. Furthermore, the 960 bp sequence also shared over 400/'0 sequence homology to rat vasopressin, and MIH and CHH of some crustaceans. We called this sequence the Novel Peptide Sequence (NPS). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。