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題名 | 利用反轉錄聚合酶鏈反應技術在新城雞病病毒臺灣分離株之檢測=Detection of Newcastle Disease Virus Isolates in Taiwan Using the Reverse Transcription-Polymerase Chain Reaction |
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作者 | 林德田; 許天來; 林地發; 鄭明珠; 楊華章; 黎南榮; 蘇杰夫; 林士鈺; | 書刊名 | 臺灣省家畜衛生試驗所研究報告 |
卷期 | 34 1998.12[民87.12] |
頁次 | 頁43-51 |
分類號 | 437.717 |
關鍵詞 | 新城雞病; 反轉錄--聚合酶鏈反應; Newcastle disease; Reverse transcription-polymerase chain reaction; |
語文 | 中文(Chinese) |
中文摘要 | 為發展快速精確診斷新城雞病,並設計二組共4條配對引子,應用反轉錄聚合 �t反應技術(Reverse transcription polymerase chain reaction, RT-PCR)以檢測新城雞 病病毒 (Newcastle disease virus, NDV), 所得 PCR 產物以快速電泳分析與 ethidium bromide 染色證實為新城雞病病毒核酸之特異增幅產物,因此本技術可應用於新城雞病病毒 之檢測。利用 RT-PCR 技術檢測於 1997 ∼ 1998 年間本省臺南縣、彰化縣、桃園縣、桃園 縣、苗栗縣及新竹縣等縣市之疑似新城雞病病例,結果可快速檢測出 DN 陽性的病材及產生 特異 PCR 增幅產物,因此本技術可應用在疑似新城雞病病例初步快速診斷參考。 |
英文摘要 | For the development of a rapid diagnostic technique of Newcastle disease, two primer pairs were designed and used in the reverse transcription polymerase chain reaction (RTPCR) for the detection of Newcastle disease virus (NDV). Amplified cDNA of NDV was confirmed by electrophoresis and staining of ethidium bromide. The technique was further applied to the diagnosis of suspected Newcastle cases occurring in Prefectures of Tainan, Chuanghwa, Taoyuang, Mialee and Hsingchu during January 1997 to August 1998. Specific PCR products could be amplified and confirmed in all cases of Newcastle disease which were diagnosed by routine virus isolation and identification. The RT-PCR technique developed could be used first and before any other type of examination for the rapid, tentative diagnosis of Newcastle disease. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。