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題 名 | 吳郭魚肝臟粒線體呼吸作用及其受到鎘離子影響之探討=A Study on the Respiration of Tilapia Liver Mitchondria and the Effect of Cadmium Ions on it |
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作 者 | 李安進; 秦宗顯; | 書刊名 | 嘉義技術學院學報 |
卷 期 | 60 1998.10[民87.10] |
頁 次 | 頁89-98 |
分類號 | 438.661 |
關鍵詞 | 粒線體; 腺核甘二磷酸依賴型呼吸作用; 鎘; Mitochondria; ADP-dependent respiration; Cadmium; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究自吳郭魚(Oreochromis mossambicus)肝臟,分離出具高度完整性的粒線體 並探討其呼吸作用。 粒線體的完整性皆在 90 %以上, 以丙酮酸 (pyruvate) 和蘋果酸 (malate) 為受質測得粒線體狀態 3 耗氧率約為 28 O �� nmol/mg/min, 而狀態 4 的耗氧 率約為 7 O �� nmol/mg/min,因此其呼吸控制分率 (RCR) 為 4;並進一步求得粒線體合成 90 μ M ATP 須消耗 15 μ M 氧分子,因此其磷氧比值 (P/O ratio) 為 3。另外以不同濃 度的鎘離子對粒線體呼吸作用做毒性測試時, 發現狀態 3 的耗氧率隨著鎘離子濃度的增加 而降低,由 28 O �� nmol/mg/min 降至 16 O �� nmol/mg/min,然而狀態 4 的耗氧率卻隨 著鎘離子濃度的增加而增加,由 8 O �� nmol/mg/min 增至 16 O �� nmol/mg/min,其 RCR 值由 3.3 降至 1。 因此推論鎘離子對粒線體的毒性作用,乃在高濃度時增加內膜對氫質子 的通透性,導致粒線體的產能作用下降。 |
英文摘要 | Mitochondria isolated from tilapia (Oreochromis mossambicus) liver with highly intact outer membrane (intactness>90%) was used for this study. In the case of pyruvate and malate as substrates, the oxygen consumption rates of state Ⅲ and state Ⅳ of liver mitochondria were 28 and 7 O �� nmol/mg/min, respectively. Therefore, the respiratory control ratio (RCR) was 4. Fifteen μ M of oxygen molecules were consumed during the syntheis of 90 μ M of ATP by using pyruvate and malate as substrates. The P/O ratio was counted as 3. By the same time, Cadmium ions were used to test their toxicities on the mitochondrial respiration. It was found that cadmium ions decrease the oxygen consumption rate of state Ⅲ from 28 to 16 O �� nmol/mg/min. However, they increased the oxygen consumption rate of state Ⅳ from 8 to 16 O �� nmol/mg/min. The value of RCR was decreased from 3.3 to 1 by cadmium ions. Therefore, cadmium ions may increase the permeability of the mitochondrial inner membrane to protons at high concentration. This resulted in a decrease in the energy production of mitochondria. |
本系統中英文摘要資訊取自各篇刊載內容。