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題名 | Animal Model of Lung Cancer Metastasis: Comparison of Efficiency of Progpagating Human Lung Cancer Cell Lines in SCID Mice with Intrabronchial, Percutaneous Intrathoracic, and Subcutaneous Routes=肺癌轉移動物模式(SCID Mice)、比較支氣管內注射、經皮穿胸注射和皮下注射人類肺癌細胞株有何不同 |
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作者 | 賴吾為; 陳芬芬; 陳肇寅; 劉校生; | 書刊名 | 中華民國外科醫學會雜誌 |
卷期 | 31:2 民87.03-04 |
頁次 | 頁90-96+96_1-96_2 |
分類號 | 415.138 |
關鍵詞 | 肺癌; 自發性轉移; 動物模式; 人類肺癌細胞株; |
語文 | 英文(English) |
中文摘要 | 建立一「肺癌」「自發性轉移」的「動物模式」是用來研究肺癌生長和轉移的良好 工具,我們嘗試使用五種不同的「人類肺癌細胞株」(SK-LU-1, Calu-1, H2981, NCI-Hut 125, CH-27 LC-1)和SCID老鼠來建立肺癌自發性轉移的動物模式。體外實驗我們用soft agar來 檢測各細胞株群落(colony)的形成能力。而SCID老鼠體內實驗用三種方式,分別是支氣管 內細胞注射,經皮穿胸注射,皮下注射。我們發現H2981腺癌細胞不僅在體外有最強的群 落形成能力,在SCID老鼠身上也顯現最強的腫瘤形成力。實驗結果顯示皮下注射所形成的 腫瘤都被周組織包�埵磽茖S有轉移現象。經皮穿胸注射所形成的腫瘤都僅波及肋膜腔和胸壁 而沒侵犯到肺組織而支氣管內細胞注射那一組,若有肺內腫瘤形成,則約66%(4/6)可以同 時發現縱膈腔淋已經轉移,和人類肺癌轉移很類似,所以本實驗的是將人類肺癌細胞株在 SCID老鼠身上經由支氣管內注射模式是研究肺癌自發生轉移可行方法。 |
英文摘要 | An ideal animal model for spontaneous metastasis of human lung cancer may provide better insight into the growth, progression, and metastasis of cancer cells, as well as an opportunity to develop methods for its control. Five human lung cancer cell lines (SK-LU-1, Calu-1, H2981, NCI- Hut 125, and CH-27 LC-1) were selected in an attempt to develop such a model. The tumorigenic potential of these cells was initially assessed by their ability to form colonies in semisolid soft agar (0.33%). Subsequently, SCID mice were inoculated with these cell lines through three different routes, i.e. intrabronchial (i.b. instillation, percutaneous intrathoracic (p.t.), and subcutaneous (s.c.), and the results were evaluated. Human adenocarcinoma cell line H2981 formed colonies in semisolid soft agar and correspondly, showed higher tumorigenic potential in SCID mice. The tumors formed by s.c. implantation were encapsulated and led to no metastasis. The percentage of tumor formation of five human lung cancer cell lines in SCID mice with p.t. implantation were 60% (3/5) for H2981, 40% (2/5) for NCI-Hut 125 and no growth for the other three cell lines. Extensive pleural and chest wall involvements without lung parenchyma lesion or metastasis resulted from p.t. implantation. On the contrary, the mice inoculated through the i.b. route had lung parenchyma tumors and regional mediastinal lymph node metastases were noted in 66% (4/6) of tumor-bearing mice. In conclusion, inoculation of SCID mice through the i.b. route stimulates tumor formation similar to lung cancer formation in human patients. Thus, this method is useful for in vivo studies of spontaneous metastatic biology and experimental therapeutics for advanced lung cancer. |
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