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題 名 | Dideoxy Fingerprinting for Rapid Screening of RpoB Gene Mutations in Clinical Isolates of Mycobacterium Tuberculosis=利用dideoxy fingerprinting快速篩檢自臨床檢體分離之結核菌rpoB基因之突變 |
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作 者 | 劉永慶; 黃采菽; 黃文貴; 陳綺鄉; 竇慧琴; | 書刊名 | 臺灣醫學會雜誌 |
卷 期 | 97:6 1998.06[民87.06] |
頁 次 | 頁400-404 |
分類號 | 414.83 |
關鍵詞 | 篩檢; 臨床; 檢體分離; 結核菌; rpoB基因; 突變; Dideoxy fingerprinting; Rifampin resistance; Mycobacterium tuberculosis; |
語 文 | 英文(English) |
英文摘要 | Rifampin is a key component of therapeutic regimens for tuberculosis control, and a marker for multidrug resistance of Mycobacterium Mutations responsible for conferring rifampin resistance in M. tuberculosis are known to occur in a 69-bp region of the rpoB gene. In this study, we assessed the accuracy of dideoxy fingerprinting (ddF), a hybrid technique employing elements of dideoxy sequencing and single-strand polymorphism analysis, for rapid screening of rifampin resistance in clinical isolates of M. tuberculosis. This technique was used to analyze 72 M. tuberculosis isolates. The results were compared with those of automated dideoxy sequencing and the antibiotic resistance profile (determined with the BACTEC system). Of the 72 isolates, 50 were rifampin resistant. The ddF findings were completely consistent with those of dideoxy sequencing in all isolates. In 68 (94%) isolates, the ddF findings were consistent with the rifampin resistance status determined with the BACTEC system; all four isolates with inconsistent results had no mutation in the 69-bp region, but were resistant to rifampin. Our findings suggest that ddF accurately detects mutations in the rifampin resistance-associated 69-bp region of the rpoB gene in clinical isolates of 24. tuberculosis, and may be a valuable screening tool for rifampin resistance. |
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