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來源資料
頁籤選單縮合
題名 | Line Probe Assay for Rapid Detection of Mutations in the rpoB Gene of Mycobacterium Tuberculosis=以直線探針分析法快速偵測結核菌rpoB基因之突變 |
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作者姓名(中文) | 劉永慶; 黃采菽; 黃文貴; | 書刊名 | 臺灣醫學會雜誌 |
卷期 | 98:8 1999.08[民88.08] |
頁次 | 頁582-585 |
分類號 | 415.2773 |
關鍵詞 | 直線探針分析法; 結核菌rpoB基因; Rifampin-resistant Mycobacterium tuberculosis; INNO-LiPA Rif TB assay; Line probe assay; |
語文 | 英文(English) |
英文摘要 | Detection of mutations in the rpoB gene of Mycobacterium tuberculosis has been reported to be an accurate predictor of rifampin resistance. DNA sequence analysis and screening methods such as single-strand conformation polymorphism analysis and dideoxy fingerprinting are labor-intensive, expensive, or yield results that may prove difficult to interpret. We evaluated the accuracy of a commercial line probe assay for rapid identification and characterization of mutations in the rpoB gene in 72 isolates of M. tuberculosis, including 50 rifampin-resistant and 22 rifampin-susceptible strains. Ten distinct rpoB mutations were identified. Concordances with automated sequencing results and phenotypic rifampin susceptibility testing results were 99% and 93%, respectively. The results demonstrate the line probe assay to be a rapid (as short as 1 day) and informative tool for the early detection and characterization of rpoB mutations associated with rifampin resistance in a clinical laboratory setting. |
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