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| 題 名 | Detection of Tetrodotoxin by High Performance Liquid Chromatography in Lined-Moon Shell and Puffer Fish=高效液相層析應用於細紋玉螺與河豚之河豚毒素檢測 |
|---|---|
| 作 者 | 陳治宇; 周宏農; | 書刊名 | Acta Zoologica Taiwanica |
| 卷 期 | 9:1 1998.01[民87.01] |
| 頁 次 | 頁41-49 |
| 分類號 | 438.661 |
| 關鍵詞 | 河豚毒; 細紋玉螺; 虎河豚; 六斑二齒魨; Tetrodotoxin; Natica lineata; Takifugu rubripes rubripes; Diodon holocanthus; |
| 語 文 | 英文(English) |
| 中文摘要 | 本研究建立一套利用管柱後反應衍生螢光產物的高效液相層析與樣品前處理的操作,應用於細紋玉螺、刺河豚與虎河豚之河豚毒定量分析。所採用的前處理操作具有91%之回收率,而河豚毒的層析檢測在10~2000ng的樣品範圍內,在測得的訊號反應與毒素濃度間呈現良好的線性關係。由於高效液相層析定量分析的結果經轉換成老鼠單位與老鼠毒性試驗所得結果間存在著極佳之相關,又毒量的檢測極限可達10ng,適用於單隻貝體或極少魚體組織樣品之毒性分析。在全年性的分月採樣分析屏東地區細紋玉螺的河豚毒含量及含毒個體數,發現其有毒樣品比例佔有45%,各月均有有毒樣品而不具季節性的偏好。養殖虎河豚的肝臟、卵巢及肌肉皆未能檢測出河豚毒的存在,但野生的六斑二齒魨卵巢內含有微量毒素,而肌肉並未含毒。 |
| 英文摘要 | High performance liquid chromatography (HPLC) using fluorescent detection following post-column alkaline degradation and a sample preparation procedure for the analysis were established to quantitatively detect tetrodotoxins (TTXs) in gastropods and puffer fishes. The analysis showed 91% recovery of TTXs using the sample preparation described in this article and a wide (10-2000 ng) linear relationship between the tetrodotoxin amount and its fluorescent response. A good correlation between the results of mouse bioassay and HPLC was also obtained in this research. Each specimen of lined-moon shell, Natica linea-ta Roeding, collected from Tungkang from October 1994 to September 1995 was analyzed by HPLC to detect the presence and quantity of TTXs. Overall results showed that 45% of the gastropods contained TTXs with an average content of 54.5μg in toxic specimens. The rate of toxic specimens in the ten samples collected monthly varied, but there were no sea-sonal trends. Random sampling of cultured tiger puffer, Takifugu rubripes rubripes Temminck & Schlegel, and field-collected balloonfish, Diodon holocanthus Linnaeus, trace amounts of TTXs were found only in ovaries of balloonfishes. Muscle tissues of balloon-fishes, and the livers, ovaries, and muscles of cultured tiger puffers showed no tetrodotoxin (< 0.2 μg/g tissue) in this screening procedure. |
本系統中英文摘要資訊取自各篇刊載內容。