查詢結果分析
相關文獻
- In Vitro and in Vivo Proliferation and Differentiation of the Virulent RH Strain of Toxoplasma Gondii Tachyzoites
- 凍解及新鮮豬黃體細胞對激黃體物質於體外分泌孕酮之效應
- 影響斜夜蛾細胞株生產桿狀病毒之因子
- 進行性肌肉萎縮症中藥療效之評估--肌胚細胞培養及動物實驗之研究(第一年)
- 宜蘭縣南澳鄉與南投縣仁愛鄉泰雅族原住民和當地動物弓蟲抗體之血清流行病學研究
- 生技藥品工廠建廠設計
- Failure of Screening to Detect HIV in a Foreign Laborer Who Died of Toxoplasmosis of the Central Nervous System
- 豬眼虹膜色素上皮細胞之分離與培養
- 臺灣東部原住民地區家犬感染弓蟲症之血清流行病學調查
- 細胞培養中黴漿菌污染之檢測與去除
頁籤選單縮合
題 名 | In Vitro and in Vivo Proliferation and Differentiation of the Virulent RH Strain of Toxoplasma Gondii Tachyzoites=弓蟲強毒RH株Tachyzoites在體外及體內之增殖與分化 |
---|---|
作 者 | 林大盛; 詹逞洲; | 書刊名 | Acta Zoologica Taiwanica |
卷 期 | 9:1 1998.01[民87.01] |
頁 次 | 頁19-32 |
分類號 | 415.291 |
關鍵詞 | 細胞培養; 弓蟲; Tachyzoite; Bradyzoite; Cell culture; T. gondii; |
語 文 | 英文(English) |
中文摘要 | 弓蟲囊胞破裂為造成愛滋病(AIDS)患者進行性弓蟲症之主因。雖然弓蟲弱毒株能夠在小鼠腦內形成囊胞,其所造成的傷害並不如在有弓蟲性腦炎之AIDS患者嚴重。目前對於tachyzoite-bradyzoite相互轉變之機制仍不是很清楚。在本研究,吾人要探討弓蟲強毒株RH株tachyzoite在體外及體內兩種條件之增殖與分化。兔抗弓蟲血清及calcium ionophore A23187/phorbol myristate acetate刺激之小鼠淋巴上清液乃用來抑制tachyzoites之過度增殖。此上清液以sandwich ELISA證實含有IFN-g。兔抗血清抗體力價可達216且以immunoblotting法可辨識主要之tachyzoite 65、45、40、30及20 kD抗原。當使用正常兔血清,可看到一非常微弱之40 kD帶。在小鼠腹腔巨噬細胞之培養中加入5%兔抗血清及5%淋巴上清液,於tachyzoites感染一週後,弓蟲會被侷限在細胞內且無進一步之細胞破壞。當抗血清或淋巴上清液停止供應,或加入兔抗IFN-g血清,細胞內弓蟲會再度增殖。本實驗並沒有證實侷限在細胞內蟲體有bradyzoite特異性抗原。然而,tachyzoite特異性30 kD蛋白質之消失以及抗pepsin消化之特性,說明了tachyzoites分化成似bradyzoites蟲體。將RH tachyzoites注射入先以弓蟲抗原免疫過之小鼠腹腔,再於不同時間給予抗血清及淋巴上清液,小鼠最後仍會死亡。在感染的小鼠腦內,以顯微鏡觀察並沒發現有囊胞,但以polymerase chain reaction證實弓蟲之存在。 在小鼠腦內之磨乳劑,因對pepsin消化沒抗性,且有tachyzoite特異性30 kD抗原,故判斷蟲體仍然為tachyzoites。 |
英文摘要 | Rupture of Toxoplasma gondii cysts has been implicated as the cause of progressive toxo-plasmosis in patients with acquired immunodeficiency syndrome (AIDS). Although non-virulent strains of T. gondii are able to form cysts in mouse brains, lesions are not as severe as those found in AIDS patients with toxoplasmic encephalitis. The mechanism of tachy-zoite- bradyzoite interconversion is still poorly understood. In this study, we examine the proliferation and differentiation of the virulent RH strain of T. gondii tachyzoites under both in vivo and in vitro conditions. Rabbit anti-T. gondii serum and calcium ionophore A23187/phorbol myristate acetate-stimulated mouse lymphocyte supernatant were used to inhibit overgrowth of tachyzoites. The supernatant contained interferon gamma (IFN-γ ) as proven by sandwich ELISA. Rabbit antiserum had antibody titers up to 2 16 and recognized major tachyzoite antigens of 65, 45, 40, 30, and 20 kD as shown by immunoblotting. A very weak 40-kD band was visualized when normal rabbit serum was used. In the presence of both 5% antiserum and 5% lymphocyte supernatant in mouse peritoneal macrophage cultures, T. gondii organisms were confined intracellularly and no further cells were destroyed at 1 week post-inoculation. When antiserum and/or lymphocyte supernatant were not supplied or rabbit anti-IFN-γ serum was added, intracellular T. gondii multiplied again. The present study did not demonstrate the presence of bradyzoite-specific antigens in the intracellularly confined organisms. However, the disappearance of the tachyzoite-specific 30-kD protein and resistance to pepsin digestion suggest the occurrence of differen-tiation from tachyzoites to bradyzoite-like organisms. When RH tachyzoites were inoculat-ed i.p. into mice, mice eventually died even if they were immunized with T. gondii antigens, and were later given antisera and/or lymphocyte supernatants at different periods. In the brains of infected mice, cysts were not found by microscopy, but the presence of T. gondii was proven by polymerase chain reaction. Organisms in mouse brain homogenates remained as tachyzoites, because they were sensitive to pepsin digestion and had tachyzoite-specific 30-kD antigen. |
本系統中英文摘要資訊取自各篇刊載內容。