查詢結果分析
相關文獻
- 放射線引起的惡性纖維組織細胞瘤在第三和第十一對染色體之異合性喪失--病例報告
- Primary Malignant Fibrous Histiocytoma in Uremic Kidney: A Case Report and Literature Review
- Retroperitoneal Malignant Fibrous Histiocytoma: Case Analyses and Literature Review
- Primary Malignant Fibrous Histiocytoma of the Lung: A Case Report
- Angiomatoid Malignant Fibrous Histiocytoma--A Case Report
- Malignant Fibrous Histiocytoma of the Maxillary Sinus Presenting as Toothache
- Perforated Malignant Fibrous Histiocytoma of the Colon Presented as Liver Abscess: Report of a Case
- Malignant Fibrous Histiocytoma of the Mesentery: A Case Report
- Primary Malignant Fibrous Histiocytoma of the Pleura: Report of a Case
- Malignant Fibrous Histiocytoma-Analysis of 44 Cases
頁籤選單縮合
題名 | 放射線引起的惡性纖維組織細胞瘤在第三和第十一對染色體之異合性喪失--病例報告=Loss of Heterozygosity on Chromosomes 3 and 11 in a Radiation-Induced Malignant Fibrous Histiocytoma--Case Report |
---|---|
作者 | 柯政郁; 周玉山; 陳培哲; 徐茂銘; Ko, Jenq-yuh; Jou, Yuh-shan; Chen, Pei-jer; Hsu, Mow-ming; |
期刊 | 中華民國耳鼻喉科醫學會雜誌 |
出版日期 | 19980200 |
卷期 | 33:1 1998.02[民87.02] |
頁次 | 頁86-91 |
分類號 | 415.138 |
語文 | chi |
關鍵詞 | 惡性纖維組織細胞瘤; 失異合性; 微衛星多形標記; Malignant fibrous histiocytoma; Loss of heterozygosity; Microsatellite polymorphic marker; |
中文摘要 | 癌症的形成大都是一連串的基因變化所致, 其中抑癌基因的不活化是癌細胞形成 最常見的現象,雖然抑癌基因的不活化有很多機轉,找出腫瘤細胞失去的染色體片段,再從 中找尋可能含有的抑癌基因是一種有效的方法。來自雙親的兩個同源染色體,若其中之一有 一段缺失,謂之有失異合性。部份頭頸部癌症在第 3,9,11 對染色體已被發現有失異合性 。頭頸部區域之放射線引起的惡性纖維組織細胞瘤,是一種惡性度極高的肉瘤,和原發性的 不一樣,前者非常罕見但生長極為快速,復發率高。為瞭解放射線引起的惡性纖維組織細胞 瘤在第 3,9,11 對染色體有無失異合性的情形, 取 1 名惡性纖維組織細胞瘤病人的癌組 織,及抽取周邊血液,分別萃取癌細胞及正常淋巴球之去氧核醣核酸,然後以位於第 3, 9 , 11 對染色體上特定位置的 53 個附有螢光物質的微衛星多形標記引子,放入分別含有正 常細胞或癌細胞去氧核醣核酸的反應液中,藉助聚合酵素鏈反應複製 60 次,確定有產物後 ,便在 Perkin-Elmer/Applied Biosystems ( PE/ABI )的 337 去氧核醣核酸分析儀進行 電泳,經雷射激發,蒐集訊息於電腦, 再以 GeneScan �薱n體判讀。 結果在染色體 3p 的 D3S1300 和 3q 的 D3S1262、D3S1311 及染色體 11p 的 D11S922 有失異合性的情形。 |
英文摘要 | Malignant transformation in tumor development results from a series of genetic changes and the inactivation of tumor suppressor genes is among the most common molecular events that contribute to tumorigenesis. Although a variety of mechanisms exsist for the inactivation of tumor suppressor genes, loss of chromosomal segments in tumor cells has proven common and useful in mapping regions containing putative tumor suppressor genes. Loss of one homologous chromosomal segment means loss of heterozygosity (LOH) (hemizygous deletion). Microsatellite polymorphic markers consist of CA dinucleotide repeats and often display extensive length polymorphism. LOH on the chromosomes 3, 9, 11 by means of microsatellite markers has been found in squamous cell carcinomas of the head and neck. The radiation-induced malignant fibrous histiocytoma in the head and neck region is rare and grows very rapidly with a poor prognosis. To detect LOH in this cancer, we analysed microsatellite polymorphism in the tumor tissue and peripheral lymphocytes of a patient with radiation-induced malignant fibrous histiocytoma. Fifty-three microsatellite polymorphic markers with corresponding pairs of primers labelled with fluorescent on chromosomes 3, 9 and 11 were used. The respective DNA's of the cancer cells and normal lymphocytes were amplified with polymerase chain reaction (PCR). We analysed the PCR products on a Model 377 DNA Analysis System (Perkin-Elmer/Applied Biosystems Division) using the GeneScan�� software. LOH was found on one marker of chromosome arm 3p (D3S1300), two markers of chromosome arm 3q (D3S1262, D3S1311), and one marker of chromosome arm 11p (D11S922). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。